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Series GSE129093 Query DataSets for GSE129093
Status Public on Apr 22, 2019
Title IL-21/type I interferon interplay regulates neutrophil-dependent innate immune responses to Staphylococcus aureus
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Methicillin-resistant Staphylococcus aureus (MRSA) is a major hospital- and community-acquired pathogen, but the mechanisms underlying host-defense to MRSA remain poorly understood. Here, we investigated the role of IL-21 in this process. When administered intra-tracheally into wild-type mice, IL-21 induced granzymes and augmented clearance of pulmonary MRSA but not when neutrophils were depleted or a granzyme B inhibitor was added. Correspondingly, IL-21 induced MRSA killing by human peripheral blood neutrophils. Unexpectedly, however, basal MRSA clearance was enhanced when IL-21 signaling was blocked, both in Il21r KO mice and in wild-type mice injected with IL-21R-Fc fusion-protein. This correlated with increased type I interferon and an IFN-related gene signature, and indeed anti-IFNAR1 treatment diminished MRSA clearance in these animals. Moreover, we found that IFN╬▓ induced granzyme B and promoted MRSA clearance in a granzyme B-dependent fashion. These results reveal an interplay between IL-21 and type-I IFN in the innate immune response to MRSA.
 
Overall design RNA-Seq analysis using total RNA either from total lung tissues isolated from mouse or from peripheral blood purified from normal donors or AD-HIES patients.
 
Contributor(s) Spolski R, West EE, Li P, Veenbergen S, Yung S, Kazemian M, Oh J, Yu Z, Freeman AF, Holland SM, Murphy PM, Leonard WJ
Citation(s) 30969166
Submission date Mar 31, 2019
Last update date Oct 09, 2019
Contact name Peng Li
E-mail(s) peng.li@nih.gov
Organization name NIH
Department NHLBI
Lab LMI
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (3)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (28)
GSM3693156 RNA-Seq WT PBS 7h
GSM3693157 RNA-Seq WT IL21 7h
GSM3693158 RNA-Seq WT PBS 24h
Relations
BioProject PRJNA530063
SRA SRP189956

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129093_RAW.tar 20.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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