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Series GSE129064 Query DataSets for GSE129064
Status Public on Mar 29, 2019
Title Time course RNA-seq profiling of mouse sorted Pdgfra+ fibroblasts
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The respiratory system undergoes remarkable structural, biochemical, and functional changes necessary for adaptation to air breathing at birth. To identify dynamic changes in gene expression in the diverse pulmonary cells at birth, we performed Drop-seq based massive parallel single-cell RNA sequencing. An iterative cell type identification strategy was used to unbiasedly identify the heterogeneity of murine pulmonary cell types on several time points. Cell type predictions and signature genes identified using Drop-seq were cross-validated using an independent single cell isolation platform. Temporal changes in RNA expression patterns were compared before and after birth to identify signaling pathways selectively activated in specific pulmonary cell types, demonstrating activation of UPR signaling during perinatal adaptation of the lung. Present data provide the first single cell view of the adaptation to air breathing after birth. All data from the present study are freely accessed at
Overall design Time course RNA-seq profiling of mouse sorted Pdgfra+ fibroblasts
Contributor: LungMAP Consortium
Contributor(s) Xu Y, Whitsett JA, Perl AK
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NIH grant(s)
Grant ID Grant title Affiliation Name
U01 HL122642 "Lung MAP" Atlas Research Center CHILDREN'S HOSPITAL MEDICAL CENTER Jeffrey A Whitsett
Submission date Mar 29, 2019
Last update date Nov 10, 2020
Contact name LungMAPP DCC
Organization name LungMAP DCC
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM3692482 E18.5 Pdgfra+ fibroblasts
GSM3692483 E16.5 Pdgfra+ fibroblasts
GSM3692484 P07 Pdgfra+ fibroblasts
This SubSeries is part of SuperSeries:
GSE128810 LungMAP Consortium Data
GSE129151 CCHMC LungMAP Consortium Data
BioProject PRJNA529890
SRA SRP189889

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129064_LMEX0000001221_exprMatrix_final.txt.gz 350.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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