GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE128822 Query DataSets for GSE128822
Status Public on Mar 03, 2020
Title High-dimensional single cell analysis reveals an effector Treg molecular program that correlates with lung cancer progression
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Anti-tumor immune responses are counteracted by CD4+ regulatory T (Treg) cells in the tumor micronenvironment, but the molecular mechanisms responsible for the enhanced suppressive activity of Tregs are poorly defined. High-dimensional single cell profiling of millions of single CD4+ and CD8+ T cells from 53 chemotherapy-na‹ve individuals with lung adenocarcinoma identified the transcription factor IRF4 as constitutively expressed by effector CD4+ Tregs present exclusively in tumors. These IRF4+, but not the quiescent IRF4_ÿTregs expressed a vast array of suppressive molecules, positively correlated with multiple inhibitory and exhausted subpopulations of T cells and were more abundant in rapidly progressing patients. Integration of transcriptomic data and whole genome binding sites of transcription factors further revealed that IRF4, either alone or in combination with its partner BATF, directly controlled a molecular program responsible for Treg hyperproliferation and suppressive functions that included CTLA-4, ICOS, TNFRSF9 (4-1BB) and IL1R2, previously shown to impact survival in lung cancer. Thus, interfering with the IRF4-dependent molecular program may represent a novel immunotherapeutic approach capable to block immunosuppression in the tumor microenvironment without inducing autoimmunity in peripheral tissues.ÿ
Overall design Transcriptional profiling of human tumor infiltrating Tregs. Gene expression profile of facs sorted Tregs (CD4+CD25+CD127-CCR8+ICOS+ and CD4+CD25+CD127-CCR8-ICOS-) from 5 surgically resected and cryopreserved human non small-cell lung cancer carcinomas.
Contributor(s) Alvisi G, Puccio S, Brummelman J, Paoluzzi Tomada E, Mazza E, Lugli E
Citation(s) 32125291, 34552178
Submission date Mar 25, 2019
Last update date Oct 13, 2021
Contact name Simone Puccio
Organization name Humanitas Clinical and Research Center
Lab Translational Immunology Lab
Street address Via Alessandro Manzoni, 56
City Rozzano
State/province Milan
ZIP/Postal code 20089
Country Italy
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (10)
GSM3686469 T111_CCR8minus_ICOSminus
GSM3686470 T111_CCR8plus_ICOSplus
GSM3686471 T124_CCR8minus_ICOSminus
BioProject PRJNA528966
SRA SRP189378

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128822_ICOSpCCRp_ICOSmCCR8m_raw_counts.csv.gz 255.0 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap