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Series GSE12856 Query DataSets for GSE12856
Status Public on Sep 20, 2008
Title Penetration resistance: Wildtype and ataf1-1 mutant response to Bgh 12 h after inoculation, 2*2 factorial design
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Arabidopsis is a non-host to the biotrophic fungus Blumeria graminis f.sp. hordei (Bgh), effectively hindering Bgh ingress to the epidermal cells by deposition of callosic papillae formations, i.e. penetration resistance. To better understand the transcriptional changes in Arabidopsis towards Bgh, we compared un-inoculated and inoculated wild-type Arabidopsis samples, with those an ATAF1 mutant allele, compromised in penetration resistance. The experiment included sampling of 8 rosettes for each replicate sample from 6-weeks old plants, 12 hrs after inoculation. A total of 12 biolocigal replicates were sampled with three replicates from each of the four blocks (Wild-type, ataf1-1, Bgh, control). ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Michael Krogh Jensen. The equivalent experiment is AT31 at PLEXdb.]
 
Overall design genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Un-inoculated(3-replications); genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Bgh inoculated(3-replications); genotype: ataf1-1 mutant, ecotype Col-0 - treated or untreated: Un-inoculated(3-replications); genotype: ataf1-1 mutant, ecotype Col-0 - treated or untreated: Bgh inoculated(3-replications)
Web link http://www.plexdb.org/modules/PD_browse/experiment_browser.php?experiment=AT31
 
Contributor(s) Jensen MK
Citation(s) 18694460
Submission date Sep 19, 2008
Last update date Aug 28, 2018
Contact name PLEXdb Curator, Sudhansu Dash
Organization name Iowa State University
Department Virtual Reality Application Center
Lab PLEXdb
Street address 1023 Crop Genomes Informatics Lab (Iowa State University)
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (12)
GSM322545 genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Un-inoculated - rep1
GSM322546 genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Un-inoculated - rep2
GSM322547 genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Un-inoculated - rep3
Relations
Affiliated with GSE69995
BioProject PRJNA111089

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12856_RAW.tar 24.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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