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Series GSE128382 Query DataSets for GSE128382
Status Public on Feb 14, 2020
Title Identification and characterization of artery and vein enhancers in the human genome
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
Summary Our goal was to identify artery- and vein-specific cis regulatory elements in the human genome and use these to determine novel mechanisms that regulate arteriovenous differentiation
 
Overall design Artery and vein enhancers were identified using ChIP-Seq for K27ac and EP300 on HUAEC and HUVEC, performed in duplicate. Further analysis included RNAseq in triplicate from the same cell types. To assess binding of known transcription factors NR2F2 and ERG, we performed ChIP-Seq in duplicate samples. We assessed K27ac and K27me3 occupancy under different experimental conditions using triplicate samples from HUVEC
 
Contributor(s) Lawson N
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NIH grant(s)
Grant ID Grant title Affiliation Name
R35 HL140017 Embryonic origins of endothelial heterogeneity UNIV OF MASSACHUSETTS MED SCHOOL NATHAN D LAWSON
Submission date Mar 15, 2019
Last update date Feb 16, 2020
Contact name Nathan D. Lawson
E-mail(s) nathan.lawson@umassmed.edu
Organization name University of Massachusetts Medical School
Department Molecular, Cell, and Cancer Biology
Street address 364 Plantation Street
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platforms (3)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL15520 Illumina MiSeq (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (58)
GSM3673407 HUAEC_H3K27ac_ChipSeq_Rep1
GSM3673408 HUAEC_InputK27acRep1
GSM3673409 HUAEC_H3K27ac_ChipSeq_Rep2
Relations
BioProject PRJNA527296
SRA SRP188557

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128382_HUVECvsHUAEC_DEseq2out.csv.gz 1.0 Mb (ftp)(http) CSV
GSE128382_NR2F2kd_DEseq2out.csv.gz 1.2 Mb (ftp)(http) CSV
GSE128382_RAW.tar 11.5 Gb (http)(custom) TAR (of BED, BIGWIG, BW, NARROWPEAK, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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