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Series GSE128176 Query DataSets for GSE128176
Status Public on May 12, 2020
Title BACH2 is repurposed following Treg lineage commitment to drive the functional quiescence and maintenance of resting Treg cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary In various tissues, cellular homeostasis is achieved by functionally quiescent stem cells which self renew while giving rise to differentiated progeny. Regulatory T (Treg) cells are composed of functionally quiescent resting Treg (rTreg) cells which differentiate into activated Treg (aTreg) cells upon antigen stimulation. How rTreg cells remain quiescent despite chronic exposure to cognate self- and foreign antigens is unclear. The transcription factor BACH2 is critical for early Treg lineage specification but its function following lineage commitment is unresolved. Here, we show Bach2 is highly expressed in lineage-committed rTreg cells but is downregulated in aTreg cells, and upon inflammation. BACH2 binds to AP-1 sites within Treg genomes and its high expression within rTreg cells functions to restrain their TCR-driven activation and differentiation into aTreg cells. As a consequence, cell-autonomous BACH2 expression is required following Treg lineage commitment for the functional quiescence and long-term maintenance of Treg cell populations and for immune homeostasis.
This SuperSeries is composed of the SubSeries listed below.
Overall design Refer to individual Series
Citation(s) 32515782
Submission date Mar 12, 2019
Last update date Aug 17, 2020
Contact name Mohammed-Firas Sadiyah
Organization name University of Cambridge
Street address The Old Schools, Trinity Ln
City Cambridge
ZIP/Postal code CB2 1TN
Country United Kingdom
Platforms (3)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (23)
GSM3636565 Foxp3-GFP+Treg_BACH2-RFP_HI_Female1_RNA-seq
GSM3636566 Foxp3-GFP+Treg_BACH2-RFP_HI_Female2_RNA-seq
GSM3636567 Foxp3-GFP+Treg_BACH2-RFP_HI_Male1_RNA-seq
This SuperSeries is composed of the following SubSeries:
GSE127695 Distribution of genome-wide BACH2 or JunD binding sites identified by ChIP-Seq in iTreg cells
GSE127698 Heterogeneous Bach2 expression distinguishes rTreg cells and aTreg cells
GSE127699 Bulk RNA-Seq analysis of WT and Bach2-deficient Foxp3+ Treg cells
BioProject PRJNA527220

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Supplementary file Size Download File type/resource
GSE128176_RAW.tar 310.0 Kb (http)(custom) TAR (of BED, NARROWPEAK)
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