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Series GSE12817 Query DataSets for GSE12817
Status Public on Jan 26, 2009
Title Cluster analysis of rat pancreatic islet gene mRNA levels after culture in low, intermediate and high [glucose]
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Background: Survival and function of insulin-secreting pancreatic β-cells are markedly altered by changes in nutrient availability. In vitro, culture in 10 rather than 2mM glucose improves rodent β-cell survival and function whereas glucose concentrations above 10mM are deleterious. Aim-Method: To identify the mechanisms of such β-cell plasticity, we tested the effects of a 18h culture at 2, 5, 10 and 30mM glucose on the transcriptome of rat islets precultured for 1 week at 10mM glucose (Affymetrix Rat 230.2 arrays). Results: Culture in either 2-5mM or 30mM instead of 10mM glucose markedly impaired β-cell function without affecting islet cell survival. Of ~16000 probe sets reliably detected in islets, ~5000 were significantly regulated at least 1.4-fold by glucose. Analysis of these probe sets with GeneCluster software identified 10 mRNA profiles with unidirectional up- or down-regulation between 2 and 10, 2 and 30, 5 and 10, 5 and 30 or 10 and 30 mM glucose, and 8 complex V-shaped or inverse V-shaped profiles with a nadir or peak level of expression in 5 or 10mM glucose. Analysis of genes belonging to these various clusters with Onto-express and GenMapp software revealed several signaling and metabolic pathways that may contribute to the induction of β-cell dysfunction and apoptosis after culture in low or high vs. intermediate glucose concentration. Conclusion: We have identified 18 distinct mRNA profiles of glucose-induced changes in islet gene mRNA levels that should help understanding the mechanisms by which glucose affects β-cell survival and function under states of chronic hypo- or hyperglycemia.

Keywords: Dose response
 
Overall design Effect of 18h culture in 2, 5, 10 and 30 mmol/l glucose on the transcriptome of rat pancreatic islets precultured for 1 week in 10 mmol/l glucose. Four experiments were done on different islet preparations over a two-months period.
 
Contributor(s) Bensellam M, Van Lommel L, Overbergh L, Schuit FC, Jonas JC
Citation(s) 19165461
Submission date Sep 17, 2008
Last update date Jul 31, 2017
Contact name Jean-Christophe Jonas
E-mail Jean-Christophe.Jonas@uclouvain.be
Phone +3227649575
Fax +3227645532
Organization name UCL, Faculty of Medicine
Department Physiology and Pharmacology
Lab Endocrinology and Metabolism
Street address Av. Hippocrate, 55
City Brussels
ZIP/Postal code B-1200
Country Belgium
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (16)
GSM322006 Pancreatic Islets_18h culture_2 mmol/l glucose_rep1
GSM322007 Pancreatic Islets_18h culture_2 mmol/l glucose_rep2
GSM322008 Pancreatic Islets_18h culture_2 mmol/l glucose_rep3
Relations
BioProject PRJNA111125

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Supplementary file Size Download File type/resource
GSE12817_RAW.tar 66.3 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file
Processed data included within Sample table

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