|
| Status |
Public on Apr 10, 2019 |
| Title |
Interrogating histone acetylation and BRD4 as mitotic bookmarks of transcription [TFs] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Global changes in chromatin organization and the cessation of transcription during mitosis are thought to challenge the resumption of appropriate transcription patterns following mitosis. The acetyl-lysine binding protein BRD4 has been previously suggested to function as a transcriptional “bookmark” on mitotic chromatin. Here, genome-wide location analysis of BRD4 in erythroid cells, combined with novel data normalization and peak characterization approaches, reveals that BRD4 widely occupies mitotic chromatin. However, removal of BRD4 from mitotic chromatin does not impair post-mitotic activation of transcription. Additionally, histone mass spectrometry reveals global preservation of most posttranslational modifications (PTMs) during mitosis. In particular, H3K14ac, H3K27ac, H3K122ac, and H4K16ac widely mark mitotic chromatin, especially at lineage-specific genes, and predict BRD4 mitotic binding genome wide. Therefore, BRD4 is likely not a mitotic bookmark but only a “passenger”. Instead, mitotic histone acetylation patterns may constitute the actual bookmarks that restore lineage-specific transcription patterns after mitosis.
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| |
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| Overall design |
Asynchronous vs nocodazole arrested mitotic cells in G1E ER4 cell line differentiated using 13h estradiol treatment
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| |
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| Contributor(s) |
Behera V, Blobel G |
| Citation(s) |
30970245 |
| |
| Submission date |
Mar 11, 2019 |
| Last update date |
May 17, 2019 |
| Contact name |
Ross Hardison |
| E-mail(s) |
rch8@psu.edu
|
| Organization name |
Pennsylvania State University
|
| Street address |
303 Wartik Lab
|
| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (15)
|
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| This SubSeries is part of SuperSeries: |
| GSE128162 |
Interrogating histone acetylation and BRD4 as mitotic bookmarks of transcription |
|
| Relations |
| BioProject |
PRJNA526569 |
| SRA |
SRP188151 |
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