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Series GSE12802 Query DataSets for GSE12802
Status Public on Jan 19, 2009
Title Small molecule inducers of pancreatic beta-cell expansion
Organism Mus musculus
Experiment type Expression profiling by array
Summary New insulin-producing pancreatic beta-cells are formed primarily by self-replication during adult life. To identify small molecules that can induce beta cell replication, a large chemical library was screened for proliferation of growth-arrested, reversibly immortalized mouse beta-cells using an automated high-throughput screening platform. A number of structurally diverse, active compounds were identified including phorbol esters, which likely act through protein kinase C, and a group of thiophene-pyrimidines that stimulate beta-cell proliferation by activating the Wnt signaling pathway. A group of dihydropyridine (DHP) derivatives was also shown to reversibly induce beta-cell replication in vitro by activating L-type calcium channels (LTCCs). Our data indicate that the LTCC agonist 2a affects the expression of genes involved in cell cycle progression and cellular proliferation. Furthermore, treatment of beta-cells with both LTCC agonist 2a and the Glp-1 receptor agonist Ex-4 showed an additive effect on beta-cell replication. The identification of small molecules that induce beta-cell proliferation suggests that it may be possible to reversibly expand other quiescent cells to overcome deficits associated with degenerative and/or autoimmune diseases.

Keywords: treatment and time course
Overall design compound treatment and time course
Contributor(s) Wang W, Walker JR, Wang X, Tremblay M, Lee JW, Wu X, Schultz PG
Citation(s) 19164755
Submission date Sep 16, 2008
Last update date Feb 11, 2019
Contact name John R Walker
Phone 858-812-1636
Organization name Genomics Institute of the Novartis Research Foundation
Lab Genetics Core
Street address 10675 John Jay Hopkins
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (16)
GSM321551 DMSO_30min
GSM321552 DMSO_2h
GSM321553 DMSO_6h
BioProject PRJNA112755

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Supplementary file Size Download File type/resource
GSE12802_RAW.tar 103.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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