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Series GSE127724 Query DataSets for GSE127724
Status Public on Mar 06, 2019
Title Neocortical projection neurons instruct inhibitory interneuron circuit development in a lineage dependent manner
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Neocortical circuits consist of stereotypical motifs that must self-assemble during development. Recent evidence suggests the subtype identity of both excitatory projection neurons (PNs) and inhibitory interneurons (INs) is important for this process. We knocked out the transcription factor Satb2 in PNs to induce those of the intratelencephalic (IT)-type to adopt a pyramidal tract (PT)-type identity. Loss of IT-type PNs selectively disrupted the lamination and circuit integration of INs derived from the caudal ganglionic eminence (CGE). Strikingly, reprogrammed PNs demonstrated reduced synaptic targeting of CGE-derived INs relative to controls. In control mice, IT-type PNs targeted neighboring CGE INs while PT-type PNs did not in deep layers, confirming this lineage-dependent motif. Finally, single cell RNA-sequencing revealed that major CGE IN subtypes were conserved after loss of IT PNs, but with differential transcription of synaptic proteins and signaling molecules. Thus, IT-type PNs influence CGE-derived INs in a non-cell autonomous manner during cortical development.
 
Overall design Single-cell RNA-seq (10X Genomics Chromium) of FAC sorted GFP+ interneurons derived from the caudal ganglionic eminence (5HT3A-GFP mouse line), from primary visual cortex of juvenile (p21) mice
 
Contributor(s) Wester JC, Mahadevan V, Rhodes CT
Citation(s) 31027966
Submission date Mar 01, 2019
Last update date Apr 05, 2022
Contact name Chris McBain
E-mail(s) mcbainc@mail.nih.gov
Phone 301-402-4778
Organization name National Institutes of Health
Department National Institute of Child Health and Human Development
Lab Cellular and Synaptic Physiology
Street address 35 Convent Drive
City Bethesda
State/province Maryland
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2)
GSM3637737 5HT3A-GFP
GSM3637738 Emx1IRES-Cre: Satb2Fl/Fl: 5HT3A-GFP
Relations
BioProject PRJNA525662
SRA SRP187709

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Supplementary file Size Download File type/resource
GSE127724_RAW.tar 29.5 Mb (http)(custom) TAR (of CSV)
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Raw data are available in SRA
Processed data are available on Series record

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