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Series GSE127695 Query DataSets for GSE127695
Status Public on May 12, 2020
Title Distribution of genome-wide BACH2 or JunD binding sites identified by ChIP-Seq in iTreg cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In various tissues, cellular homeostasis is achieved by functionally quiescent stem cells which selfrenew while giving rise to differentiated progeny. Regulatory T (Treg) cells are composed of functionally quiescent resting Treg (rTreg) cells which differentiate into activated Treg (aTreg) cells upon antigen stimulation. How rTreg cells remain quiescent despite chronic exposure to cognate self- and foreign antigens is unclear. The transcription factor BACH2 is critical for early Treg lineage specification but its function following lineage commitment is unresolved. Here, we show Bach2 is highly expressed in lineage-committed rTreg cells but is downregulated in aTreg cells, and upon inflammation. BACH2 binds to AP-1 sites within Treg genomes and its high expression within rTreg cells functions to restrain their TCR-driven activation and differentiation into aTreg cells. As a consequence, cell-autonomous BACH2 expression is required following Treg lineagecommitment for the functional quiescence and long-term maintenance of Treg cell populations and for immune homeostasis.
Overall design ChIP-seq profiles from iTreg cells, purified by FACS from WT mice, were generated by deep sequencing using Illumina HiSeq2000.
Contributor(s) Sadiyah M
Citation(s) 32515782
Submission date Mar 01, 2019
Last update date Aug 17, 2020
Contact name Mohammed-Firas Sadiyah
Organization name University of Cambridge
Street address The Old Schools, Trinity Ln
City Cambridge
ZIP/Postal code CB2 1TN
Country United Kingdom
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (3)
GSM3679892 iTreg_BACH2_ChIP-seq
GSM3679893 iTreg_JunD_ChIP-seq
GSM3679894 iTreg_Input_ChIP-seq
This SubSeries is part of SuperSeries:
GSE128176 BACH2 is repurposed following Treg lineage commitment to drive the functional quiescence and maintenance of resting Treg cells
BioProject PRJNA527352
SRA SRP188607

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Supplementary file Size Download File type/resource
GSE127695_RAW.tar 310.0 Kb (http)(custom) TAR (of BED, NARROWPEAK)
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Raw data are available in SRA
Processed data provided as supplementary file

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