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Series GSE127234 Query DataSets for GSE127234
Status Public on Aug 06, 2019
Title Transcriptome profiling of CD8+ T cells exposed to vehicle or IL-1b treatment in vivo
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNA sequencing analysis is capable of quantitatively analyzing transcriptome of a cell population at a genome-wide level. To determine the impact of systemic IL-1b administration on T cell differentiation in vivo, we adoptively transferred OT-I CD8+ T cells to a host, followed by OVA/LPS immunization and 3 daily injections of PBS or IL-1b. On day 4, OT-I cells from draining lymph nodes were sorted and the RNA was extracted for sequencing.
Overall design Compare the transcriptome profile of OT-I cells isolated from vehicle- or IL-1b-treated mice.
Contributor(s) Lee P, Gurusamy D, Restifo NP
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Submission date Feb 26, 2019
Last update date Aug 08, 2019
Contact name Devikala Gurusamy
Organization name NCI - NIH
Department Surgery Branch
Lab Restifo Lab
Street address Building 10, 3W-5816
City Bethesda
State/province Maryland
ZIP/Postal code 20852
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM3631349 Veh-1
GSM3631350 Veh-2
GSM3631351 Veh-3
BioProject PRJNA524381
SRA SRP186952

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127234_RAW.tar 1.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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