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Series GSE127005 Query DataSets for GSE127005
Status Public on Feb 26, 2019
Title Single cell transcriptomic analysis (InDrops) of mouse mesenchymal stromal cells (mSCs or FAPs, fibro-adipogenic progenitors) isolated from lymph node, muscle and visceral adipose tissue
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Regulatory T cells (Tregs) are key brakes on the VAT inflammation that regulates local and systemic metabolic tenor. The cytokine, IL-33, expands and sustains the unique Treg population residing within VAT. Making use of single-cell RNA sequencing, we identified the major IL-33 producers in VAT to be particular mSC subtypes, related to but distinct from adipocyte progenitor cells. We further characterize these subsets by individually isolating them and performing bulk-RNA sequencing. We explored modulation of the VAT-mSC (VmSC) landscape with physiologic variables such as age and sex, as well as pathogenic states like obesity. We uncovered a VAT Treg:stromal-cell negative regulatory loop that keeps the potent effect of IL-33 under rein.
Overall design Single cells were sorted by flow cytometry for single cell RNAseq library preparation (InDrops)
Contributor(s) Zemmour D, Spallanzani RG, Benoist C, Mathis D
Citation(s) 31053654
Submission date Feb 25, 2019
Last update date May 07, 2019
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (5287)
GSM3619668 mSC_LN_1
GSM3619669 mSC_LN_2
GSM3619670 mSC_LN_3
BioProject PRJNA524153
SRA SRP186786

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Supplementary file Size Download File type/resource
GSE127005_singlecellRNAseq_mSC_counts.txt.gz 7.9 Mb (ftp)(http) TXT
GSE127005_singlecellRNAseq_mSC_metadata.txt.gz 105.9 Kb (ftp)(http) TXT
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