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Series GSE126783 Query DataSets for GSE126783
Status Public on Mar 12, 2019
Title Single cell RNA-seq identifies a unique microglia subtype associated with retinal degeneration
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In many forms of retinal degenerative diseases in human, microglia relocate to and accumulate in the subretinal space. However, the roles of microglia in retinal degeneration are poorly understood. By leveraging single cell RNA-seq, we identified a distinct microglia subtype in the subretinal space. These microglia underwent transcriptional reprogramming characterized by reduced expression of homeostatic checkpoint genes and upregulation of injury-responsive genes. Importantly, this transition is associated with protection of the retinal pigment epithelium from damage caused by disease. Therefore, our data demonstrated microglial heterogeneity in retinal degeneration and may provide important implications for developing new strategies to prevent loss of vision.
 
Overall design Transcriptional profiling of Cx3cr1+ single cells from the mouse model of light-induced retinal degeneration with matched control, generated from single cell RNA-sequencing of over 10,000 cells.
 
Contributor(s) Yu C, Saban DR
Citation(s) 30850344
Submission date Feb 19, 2019
Last update date Mar 19, 2019
Contact name Daniel Saban
E-mail(s) daniel.saban@duke.edu
Organization name Duke University
Street address 2351 Erwin Rd, Duke Eye Center
City Durham
State/province NC
ZIP/Postal code 27705
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (2)
GSM3612831 ctrl
GSM3612832 LD
Relations
BioProject PRJNA523252
SRA SRP186407

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Supplementary file Size Download File type/resource
GSE126783_RAW.tar 67.0 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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