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Series GSE126584 Query DataSets for GSE126584
Status Public on Oct 21, 2019
Title Escherichia coli DH5alpha Transcriptome Sequencing under Wi-Fi radiofrequency radiation
Organism Escherichia coli
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: In this study, Escherichia coli DH5alpha whole transcriptome sequencing was performed in order to compare the different gene expression profiles between control and exposed to Wi-Fi radiofrequency radiations.
Methods:Escherichia coli DH5alpha were exposed to Wi-Fi radiations. Total RNA samples( control and exposed ) were extracted by bacteria protect-Rneasy kit,treated with DNAase and subjected to sequnecing using an Illumina-NovaSeq 6000 platform. Library preparation and sequencing were performed by Macrogen (south korea).Trimmed reads are mapped to reference genome with Bowtie. HTseq was used for expression profiling. Expression profile was calculated for each sample and gene as read count.
 
Overall design 6 samples at mid log phase including control and Wi-Fi exposed E.coli DH5α for 5 hours, in triplicate, were analyzed using Illumina NovSeq6000 plateform
 
Contributor(s) Said-Salman IH, Jebaii FA
Citation(s) 31595026
Submission date Feb 14, 2019
Last update date Oct 21, 2019
Contact name Ilham Hassan Salman
E-mail(s) ilhamsalman@hotmail.com
Organization name Lebanese university
Street address Hadath
City beirut
ZIP/Postal code 961
Country Lebanon
 
Platforms (1)
GPL25368 Illumina NovaSeq 6000 (Escherichia coli)
Samples (6)
GSM3608684 control rep1
GSM3608685 control rep2
GSM3608686 control rep3
Relations
BioProject PRJNA522450
SRA SRP185923

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE126584_Expression_Profile.GCF_002848225.1_ASM284822v1_genomic.gene.txt.gz 330.2 Kb (ftp)(http) TXT
GSE126584_RAW.tar 120.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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