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Series GSE126418 Query DataSets for GSE126418
Status Public on Mar 25, 2019
Title Effect of PTBA on acute kidney injury during AKI
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary PTBA has been published to increase renal tubular cell proliferation, increased survival, and increased renal functional recovery in fish and various models of murine models of acute kidney injury. Immunohistological analyses suggested increased cell proliferation is accompanied by increased epithelial-to-mesenchymal transition in the RTECs. In order to elucidate pathways responsible for the increased repair response after compound treatment, larval zebrafish were given AKI and treated with PTBA analogue, UPHD25 or DMSO. Results suggests that epithelial-related genes were downregulated while mesenchymal-related genes were upregulated with injury and compound treatment. Results further validate our immunohistological finding that our compound increase post-AKI repair by increasing EMT in renal tubular cells.
Overall design At 3dpf, larval zebrafish are given acute kidney injury with gentamicin microinjection. 2 days post injury, larvae with AKI are selected and treated with 1uM of PTBA analogue, UPHD25 or vehicle control, 1% DMSO. The fish were treated with UPHD25 or DMSO for 24 hours. Then, pronephric kidneys were collected using DDT, collagenase I, and manual collection. Total 100 larvae were collected per sample, per replicate. Each treatment group was repeated with 3 biological replicates. RNA was collected and sequenced.
Contributor(s) Hukriede NA, Bais AS
Citation(s) 30890583
Submission date Feb 11, 2019
Last update date Mar 25, 2019
Contact name Neil A Hukriede
Organization name University of Pittsburgh
Department Developmental Biology
Lab Hukriede
Street address 3501 Fifth Ave, 5061 BST3
City Pittsburgh
State/province PA
ZIP/Postal code 15260
Country USA
Platforms (1)
GPL20828 Illumina NextSeq 500 (Danio rerio)
Samples (6)
GSM3598105 AKI_DMSO_Rep1
GSM3598106 AKI_DMSO_Rep2
GSM3598107 AKI_DMSO_Rep3
BioProject PRJNA521808
SRA SRP185597

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Supplementary file Size Download File type/resource
GSE126418_counts.txt.gz 316.2 Kb (ftp)(http) TXT
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