|
| Status |
Public on Jul 09, 2019 |
| Title |
ChIP-Seq Analysis of Med31-FZZ |
| Organism |
Tetrahymena thermophila |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The biological samples are from Med31-FZZ tagged Tetrahymena thermophila. Whole cells extracts were crosslinked and immunoprecipitated using M2 affinity gel (Sigma). The obtained DNA was sequenced by HiSeq2500 (Illumina). The processed fastq files were analyzed by RACS that segregates the found read accumulations between genic and intergenic regions being highly efficient for rapid downstream analyses.
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| |
|
| Overall design |
Examination of 2 sets of Med31-FZZ tagged cells during vegetative growth
|
| |
|
| Contributor(s) |
Saettone A, Garg J, Ponce M, Pearlman RE, Fillingham J |
| Citation(s) |
31280994 |
| |
| Submission date |
Feb 10, 2019 |
| Last update date |
Jul 10, 2019 |
| Contact name |
Alejandro Saettone |
| E-mail(s) |
alejandro.saettonechipana@sickkids.ca
|
| Organization name |
The Hospital for Sick Children
|
| Department |
Genetics & Genome Biology
|
| Lab |
Campos Lab
|
| Street address |
686 Bay Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G0A4 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL23968 |
Illumina HiSeq 2500 (Tetrahymena thermophila) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA521656 |
| SRA |
SRP185357 |
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