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Series GSE126296 Query DataSets for GSE126296
Status Public on Feb 09, 2019
Title Sprint exercise shares gene signatures with other exercise modes and hormone exposure and induces stronger upregulation of immediate early genes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Aim: To examine the global gene expression response to the profound metabolic and hormonal stress induced by acute sprint exercise.
Methods: Healthy women and men (n=14) performed 3 all-out cycle sprints interspersed by 20 min recovery. Muscle biopsies were obtained before the first and 2h and 20 min after the last sprint. Microarray analysis was performed to analyse the acute gene expression response. Repeated blood sample were also obtained.
Results: In skeletal muscle, a set of immediate early genes, FOS, NR4A3, MAFF, EGR1, JUNB were markedly upregulated after sprint exercise. These genes were also upregulated, but to a lesser extent, after other exercise modes and after insulin exposure. Gene ontology analysis from 879 differentially expressed genes revealed predicted activation of extracellular remodelling and fat metabolism, which was in common with the gene expression response to endurance and resistance exercise. Moreover, the gene expression pattern indicated an increased turnover of skeletal muscle mass after sprint exercise, which was in common with data on resistance exercise and GH exposure. Insulin and GH may be involved in sprint-induced changes in the transcriptome, based on the overlap noted between sprint- and hormone exposure-related signatures. Furthermore, there were correlations between sprint-induced changes in hormonal levels and changes in gene expression.
Conclusion: This is the first study reporting on global gene expression in skeletal muscle in response to acute sprint exercise. Several novel findings are presented related to novel sprint exercise - regulated genes as well as to shared gene signatures between sprint exercise, other exercise modes and exogenous hormone exposure.
 
Overall design Skeletal muscle gene expression at rest and after acute sprint interval exercise (3x30-s all-out cycle sprints). The first biopsy sample was obtained randomly in either right or left leg before the first sprint. The second sample was obtained 2 h and 20 min after the third sprint from the opposite leg. Fourteen human subjects (Seven males and seven females) volunteered for the present study.
 
Contributor(s) Rundqvist HC
Citation(s) 31647849
Submission date Feb 08, 2019
Last update date Nov 12, 2019
Contact name Hakan Claes Rundqvist
E-mail(s) hakan.rundqvist@ki.se
Phone +46707333068
Organization name Karolinska institutet
Department Department of Laboratory Medicine
Lab Clinical Physiology
Street address Alfred Nobels ale 8
City Stockholm
State/province Huddinge
ZIP/Postal code 14186
Country Sweden
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (28)
GSM3595762 muscle from S1_preexercise_0min_male_1
GSM3595763 muscle from S2_preexercise_ 0min_male_1
GSM3595764 muscle from S3_preexercise_ 0min_male_1
Relations
BioProject PRJNA521490

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE126296_RAW.tar 129.5 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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