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| Status |
Public on Feb 13, 2019 |
| Title |
IFN-α-producing proliferating myeloid cells reveal type I IFN-stimulating gene signature |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We previously established a method to generate myeloid lineage cells with proliferation capacity from induced pluripotent stem cells (iPSCs) (Zhang R. Cancer Immunol Res. 3: 668, 2015). In this study, we generated the IFN-α-producing cells by genetic engineering of mouse iPSC-derived myeloid cells (iPSC-pMCs). Gene expression profiling of IFN-α-producing iPSC-pMCs (IFN-α-iPSC-pMCs) revealed a distinct interferon-stimulated gene (ISG) signature: of 613 differentially expressed genes (versus iPSC-pMC), 345 genes were ISGs of which > 70 % were type I IFN-related. Gene ontology analysis showed that the up-regulated gene signature was enriched for transcripts associate with immune responses, cellular responses to type I IFN, and defense responses to virus.
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| Overall design |
Gene expression profiles of iPSC-pMCs, IFN-α-transuduced iPSC-pMCs, and IFN-α-treated iPSC-pMCs.
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| |
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| Contributor(s) |
Uemura Y, Zhang R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 08, 2019 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Ranmaru Shimoda |
| E-mail(s) |
shimoda@rhelixa.com
|
| Phone |
+818043368250
|
| Organization name |
Rhelixa, Inc.
|
| Street address |
3F Yayoi Bldg., 3-7-4 Iwamotocho
|
| City |
Chiyoda |
| State/province |
Tokyo |
| ZIP/Postal code |
101-0032 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE126281 |
Type I Interferon Delivery by iPSC-Derived Myeloid Cells Elicits Antitumor Immunity via XCR1+ Dendritic Cells |
|
| Relations |
| BioProject |
PRJNA521844 |
| SRA |
SRP185724 |
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