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| Status |
Public on Oct 18, 2019 |
| Title |
Role of Fragaria FaPRE1 in fruit ripening [FaPRE1-RNAi] |
| Organism |
Fragaria x ananassa |
| Experiment type |
Expression profiling by array
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| Summary |
The role played by transcription factors in the regulation of strawberry fruit ripening process is scant. We have identified and functionally characterized FaPRE1, a non-DNA-binding bHLH transcription factor.
FaPRE1 is a ripening-related transcription factor that regulates genes involved in cell architecture in strawberry fruit receptacles.
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| Overall design |
A custom-made oligo microarray (60-mer length) platform (FraGenomics 35k) containing a total of 35234 singletons corresponding to those sequences published in the strawberry genome project (http://www.strawberry.org). For each sequence, 4 different oligos were printed per block and four blocks were printed per data set. We have analyzed three control samples fruits agroinjected with the empty vector, a three samples fruits agroinjected with the RNAi construct.
Please note that experiments were carried out technically as dual channel (eg, Cy3 and Cy5-labeled samples hybridized to the same array) but processed the results as though they are single channel (Cy3 and Cy5 signals are calculated; Cy3/Cy5 ratios are not calculated). Therefore 3 raw data files for total 6 samples are linked as Series supplementary files and the associated raw data file is indicated in the corresponding sample description field.
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| Contributor(s) |
Muñoz-Blanco J, Medina-Puche L, Molina-Hidalgo FJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 01, 2019 |
| Last update date |
Jan 20, 2020 |
| Contact name |
Francisco Javier Molina-Hidalgo |
| Organization name |
University of Cordoba
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| Department |
Biochemistry and molecular biology
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| Street address |
Edif. Severo Ochoa (C6.) Planta Baja-Ala Norte Campus de Rabanales
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| City |
CÓRDOBA |
| State/province |
Cordoba |
| ZIP/Postal code |
14071 |
| Country |
Spain |
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| Platforms (1) |
| GPL24234 |
037907-Agilent Fragaria x ananassa FraGenomics 35k [Feature number version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA518769 |
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