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Series GSE125855 Query DataSets for GSE125855
Status Public on Jan 30, 2019
Title Mapping polymerase usage by tracking ribonucleotide incorporation (HydEn-seq) during DNA replication
Organisms Schizosaccharomyces pombe; Saccharomyces cerevisiae
Experiment type Other
Summary We devised and improved on our hydrolytic end sequencing (HydEn-seq) that mapping ribonucleotide incorporation in genome and used this method to track DNA replicative polymerase usage. We uncovered striking exceptions to canonical polymerase division of labor in Saccharomyces cerevisiae and Schizosaccharomyces pombe.
 
Overall design Examination of location of ribonucleotides in multiple yeast strains bearing different DNA polymerase variants, deficient in rnh201.
 
Contributor(s) Zhou Z, Burkholder AB, Lujan SA, Kunkel TA
Citation(s) 31488849
Submission date Jan 29, 2019
Last update date Sep 18, 2019
Contact name Zhi-Xiong Zhou
E-mail(s) zhouzhixiong2013@gmail.com
Organization name National Institute of Envinromental Health Sciences
Street address 111 TW Alexander Dr.
City Durham
ZIP/Postal code 27709
Country USA
 
Platforms (2)
GPL21656 Illumina HiSeq 4000 (Saccharomyces cerevisiae)
GPL22682 Illumina HiSeq 4000 (Schizosaccharomyces pombe)
Samples (58)
GSM3583254 TAK_1346-S_cerevisiae_HydEn-seq_rnh201_NT
GSM3583255 TAK_1347-S_cerevisiae_HydEn-seq_rnh201_NT
GSM3583256 TAK_1351-S_cerevisiae_HydEn-seq_pol1-Y869A_rnh201_NT
Relations
BioProject PRJNA517710
SRA SRP182841

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125855_L03_ref_v2.fa.gz 3.6 Mb (ftp)(http) FA
GSE125855_RAW.tar 719.0 Mb (http)(custom) TAR (of BW)
GSE125855_oligos.fa.gz 199 b (ftp)(http) FA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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