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Series GSE12584 Query DataSets for GSE12584
Status Public on Jan 22, 2009
Title Microarray analysis of the interaction between Rhopalosiphum padi and partially resistant or susceptible barley lines
Platform organism Hordeum vulgare
Sample organisms Hordeum vulgare; Hordeum vulgare subsp. spontaneum
Experiment type Expression profiling by array
Summary The bird cherry-oat aphid (Rhopalosiphum padi L.) (Homoptera: Aphididae) is an important pest on cereals causing plant growth reduction but no specific leaf symptoms. Breeding of barley (Hordeum vulgare L.) for R. padi resistance shows that there are several resistance genes involved, reducing aphid growth. In an attempt to identify candidate sequences for resistance-related genes, we performed a microarray analysis of gene expression after two days of aphid infestation in two susceptible barley lines and two genotypes with partial resistance. One of the four lines is a descendant of two of the other genotypes. The analysis revealed large differences in gene induction between the four lines, indicating substantial variation in response even between closely related genotypes. Genes induced in the aphid-infested tissue were mainly related to defence, primary metabolism and signalling. Only twenty-four genes were induced in all lines, none of them related to oxidative stress or secondary metabolism. Few genes were down-regulated and none of those was common to all four lines. There were differences in aphid-induced gene regulation between resistant and susceptible lines, and results from control plants without aphids also revealed differences in constitutive gene expression between the two types of lines. Candidate sequences for both induced and constitutive resistance factors have been identified, among them a proteinase inhibitor, a Ser/Thr kinase and several thionins.
Overall design Four barley genotypes, two (Lina, Kara) with susceptibility to the bird cherry-oat aphid (Rhopalosiphum padi) and two (Hsp5, 28:4) with partial resistance, were infested with R. padi. After 48 h of aphid feeding, changes in transcript accumulation were analysed by comparing aphid-infested plants with control (uninfested) plants for each genotype. For each treatment, 3 biological replicates were analysed. Overlaps and differences in regulation of RNA abundance were identified between the four genotypes. 28:4 is a descendant of Hsp5 and Lina.
Contributor(s) Delp G, Gradin T, Åhman I, Jonsson LM
Citation(s) 19085010
Submission date Aug 27, 2008
Last update date Mar 20, 2012
Contact name Gabriele Delp
Phone +4686084704
Organization name Södertörns University College
Department Life Sciences
Street address Alfred Nobels allé 7
City Huddinge
ZIP/Postal code 141 89
Country Sweden
Platforms (1)
GPL1340 [Barley1] Affymetrix Barley Genome Array
Samples (24)
GSM310455 lina_with_aphids_1
GSM310863 lina_with_aphids_2
GSM310864 lina_with_aphids_3
BioProject PRJNA112637

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12584_RAW.tar 49.7 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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