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| Status |
Public on Apr 01, 2019 |
| Title |
RNA-seq data of alveolar type 2 epithelial cell derived from organoids treated with IL-1b or TNFa |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Treatment with IL-1b or TNFa enhance the proliferation of alveolar type 2 cells in organoid culture. Here, we examine the downstream mechanisms that enhance AEC2 proliferation after IL-1b and TNFa treatments, we performed RNA-seq analysis of AEC2s isolated from organoids 6 hrs after exposure to either cytokine. We identified 165 differentially expressed genes that were common in both IL-1b and TNFa treated AEC2s compared to saline treated controls.
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| Overall design |
mRNA was isolated from FACS-sorted cells and sequenced using Illumina HiSeq X
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| Contributor(s) |
Katsura H, Kobayashi Y, Rao Tata P, Hogan BL |
| Citation(s) |
30930244 |
| |
| Submission date |
Jan 25, 2019 |
| Last update date |
Apr 23, 2019 |
| Contact name |
Hiroaki Katsura |
| E-mail(s) |
hiroaki.katsura@duke.edu
|
| Organization name |
Duke University
|
| Street address |
353 Nanaline Duke Bldg
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA517119 |
| SRA |
SRP182054 |
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