|
| Status |
Public on Sep 03, 2019 |
| Title |
RACS: Rapid Analysis of ChIP-Seq data for contig based genomes |
| Organism |
Tetrahymena thermophila |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The biological samples are from untagged Tetrahymena thermophila to be used as a control for unspecific binding. Whole cells extracts were crosslinked and immunoprecipitated using M2 affinity gel (Sigma). The obtained DNA was sequenced by HiSeq2500 (Illumina). The processed fastq files were analyzed by RACS that segregates the found read accumulations between genic and intergenic regions being highly efficient for rapid downstream analyses.
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| |
|
| Overall design |
Examination of 2 sets of 2 biological replicates of untagged cells during vegetative growth
|
| |
|
| Contributor(s) |
Saettone A, Ponce M, Nabeel-Shah S, Fillingham J |
| Citation(s) |
31664892 |
| |
| Submission date |
Jan 24, 2019 |
| Last update date |
Oct 31, 2019 |
| Contact name |
Alejandro Saettone |
| E-mail(s) |
alejandro.saettonechipana@sickkids.ca
|
| Organization name |
The Hospital for Sick Children
|
| Department |
Genetics & Genome Biology
|
| Lab |
Campos Lab
|
| Street address |
686 Bay Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G0A4 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL23968 |
Illumina HiSeq 2500 (Tetrahymena thermophila) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA516862 |
| SRA |
SRP181856 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125576_FINAL.table.6_WT1_Input_S6_R1_001-5_WTB1_ChIP_S5_R1_001--SORTED.csv.gz |
610.0 Kb |
(ftp)(http) |
CSV |
| GSE125576_FINAL.table.6_WT1_Input_S86_R1_001-5_WTB1_ChIP_S85_R1_001--SORTED.csv.gz |
620.9 Kb |
(ftp)(http) |
CSV |
| GSE125576_FINAL.table.8_WT2_Input_S88_R1_001-7_WTB2_ChIP_S87_R1_001--SORTED.csv.gz |
627.5 Kb |
(ftp)(http) |
CSV |
| GSE125576_FINAL.table.8_WT2_Input_S8_R1_001-7_WTB2_ChIP_S7_R1_001--SORTED.csv.gz |
616.7 Kb |
(ftp)(http) |
CSV |
| GSE125576_tableIGR--FINAL.table.6_WT1_Input_S6_R1_001-5_WTB1_ChIP_S5_R1_001_INPUTs-IPs_20190119-200456.csv.gz |
446.3 Kb |
(ftp)(http) |
CSV |
| GSE125576_tableIGR--FINAL.table.6_WT1_Input_S86_R1_001-5_WTB1_ChIP_S85_R1_001_INPUTs-IPs_20190119-200453.csv.gz |
456.9 Kb |
(ftp)(http) |
CSV |
| GSE125576_tableIGR--FINAL.table.8_WT2_Input_S88_R1_001-7_WTB2_ChIP_S87_R1_001_INPUTs-IPs_20190119-200459.csv.gz |
468.6 Kb |
(ftp)(http) |
CSV |
| GSE125576_tableIGR--FINAL.table.8_WT2_Input_S8_R1_001-7_WTB2_ChIP_S7_R1_001_INPUTs-IPs_20190119-200501.csv.gz |
459.6 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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