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Series GSE125539 Query DataSets for GSE125539
Status Public on Feb 05, 2019
Title Gene expression profiles in double transgenic (DT, Pum2;rtTA3) and control (CTR, Pum2 and wild-type) spleens [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Although numerous long noncoding RNAs (lncRNAs) have been identified, our understanding of their roles in mammalian physiology remains limited. Here we investigated the physiologic function of the conserved lncRNA Norad in vivo. Deletion of Norad in mice results in genomic instability and mitochondrial dysfunction, leading to a dramatic multi-system degenerative phenotype resembling premature aging. Loss of tissue homeostasis in Norad-deficient animals is attributable to augmented activity of PUMILIO proteins, which act as post-transcriptional repressors of target mRNAs to which they bind. Norad is the preferred RNA target of PUMILIO2 (PUM2) in mouse tissues and, upon loss of Norad, PUM2 hyperactively represses key genes required for mitosis and mitochondrial function. Accordingly, enforced Pum2 expression fully phenocopies Norad deletion, resulting in rapid-onset aging-associated phenotypes. These findings provide new insights and open new lines of investigation into the roles of noncoding RNAs and RNA binding proteins in normal physiology and aging.
 
Overall design Gene expression profiles were determined by RNA-seq.
 
Contributor(s) Kopp F, Chen B, Zhang H, Lee S, Xie Y, Mendell JT
Citation(s) 30735131
Submission date Jan 23, 2019
Last update date Mar 22, 2019
Contact name Joshua Mendell
E-mail joshua.mendell@utsouthwestern.edu
Organization name UT Southwestern Medical Center
Street address 6000 Harry Hines Blvd., NA6.200A
City Dallas
State/province TX
ZIP/Postal code 75390-9148
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM3576758 SPL_DT1
GSM3576759 SPL_DT2
GSM3576760 SPL_DT3
Relations
BioProject PRJNA516705
SRA SRP181694

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125539_SPL_DT_TPM.txt.gz 1.1 Mb (ftp)(http) TXT
Raw data are available in SRA
Processed data are available on Series record

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