 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 23, 2019 |
| Title |
Comparative analysis reveals a role for TGF-β in shaping the residency-related transcriptional signature in tissue-resident memory CD8+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Tissue-resident CD8+ memory T (TRM) cells are immune cells that permanently reside at tissue sites where they play an important role in providing rapid protection against reinfection. They are not only phenotypically and functionally distinct from their circulating memory counterparts, but also exhibit a unique transcriptional profile. To date, the local tissue signals required for their development and long-term residency are not well understood. So far, the best-characterised tissue-derived signal is transforming growth factor-β (TGF-β), which has been shown to promote the development of these cells within tissues. In this study, we aimed to determine to what extent the transcriptional signatures of TRM cells from multiple tissues reflects TGF-β imprinting. We activated murine CD8+ T cells, stimulated them in vitro by TGF-β, and profiled their transcriptomes using RNA-seq. Upon comparison, we identified a TGF-β-induced signature of differentially expressed genes between TGF-β-stimulated and -unstimulated cells. Next, we linked this in vitro TGF-β-induced signature to a previously identified in vivo TRM-specific gene set and found considerable (>50%) overlap between the two gene sets, thus showing that a substantial part of the TRM signature can be attributed to TGF-β signalling. Finally, gene set enrichment analysis further revealed that the altered gene signature following TGF-β exposure reflected transcriptional signatures found in TRM cells from both epithelial and non-epithelial tissues. In summary, these findings show that TGF-β has a broad footprint in establishing the residency-specific transcriptional profile of TRM cells, which is detectable in TRM cells from diverse tissues. They further suggest that constitutive TGF-β signaling might be involved for their long-term persistence at tissue sites.
|
| |
|
| Overall design |
RNA-sequencing was used to profile the transcriptome of murine CD8+ T cells stimulated in vitro by TGF-β
|
| |
|
| Contributor(s) |
Nath AP, Braun A, Ritchie SC, Carbone FR, Mackay L, Gebhardt T, Inouye M |
| Citation(s) |
30742629 |
| |
| Submission date |
Jan 22, 2019 |
| Last update date |
Apr 25, 2019 |
| Contact name |
Artika Praveeta Nath |
| E-mail(s) |
natar210@gmail.com
|
| Phone |
422355575
|
| Organization name |
Baker Heart and Diabetes Institute
|
| Lab |
Systems Genomics
|
| Street address |
75 commerical road
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3004 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA516451 |
| SRA |
SRP181190 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125471_Normalised_FPKM_Values.xls.gz |
1.9 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...