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Series GSE125469 Query DataSets for GSE125469
Status Public on Sep 17, 2019
Title Effect of circPTPRM on the biological behaviors of hepatocellular carcinoma cells in vitro and its clinical significance.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In this paper, a new circRNA was discovered by RNA sequencing, and its relationship with hepatocarcinoma proliferation, apoptosis, invasion and migration and clinical correlation of hepatocellular carcinoma were verified from different angles, providing a basis for further discussion on the mechanism of promoting the occurrence and development of hepatocellular carcinoma.
Overall design RNA sequencing was performed on 3 pairs of hepatocellular carcinoma and their adjacent paired tissues, and 3 differentially expressed circRNAs were selected and verified by Real time PCR in 30 pairs of tissue samples, hepatoma cells and normal liver cells, and clinical data were analyzed. From which candidate circRNAs are selected. Then, the anti-candidate circRNA si RNA-1 and si RNA-2 were transfected into the hepatoma cell line, and the biological behavior of the hepatoma cells after silencing of the candidate circRNA was detected by cell proliferation, plate cloning, flow cytometry and transwell.
Contributor(s) Luo Z, Cui W
Citation(s) 31494761
Submission date Jan 22, 2019
Last update date Sep 17, 2019
Contact name Zhun Luo
Phone +86 18842461871
Organization name the First Affiliated Hospital, China Medical University
Department Infectious Diseases
Lab Infectious disease laboratory
Street address 155 Nanjing North Street
City Shenyang
State/province Liaoning
ZIP/Postal code 110000
Country China
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (6)
GSM3574728 DYT-C1_RNA&lncRNA&circRNA-Seq
GSM3574729 DYT-N1_RNA&lncRNA&circRNA-Seq
GSM3574730 HXR-C1_RNA&lncRNA&circRNA-Seq
BioProject PRJNA516450
SRA SRP181192

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Supplementary file Size Download File type/resource
GSE125469_All_GSE125469_Differentially_GSE125469_Expressed_Genes.xlsx 3.1 Mb (ftp)(http) XLSX
GSE125469_All_GSE125469_Differentially_GSE125469_Expressed_circularRNA.xlsx 617.7 Kb (ftp)(http) XLSX
GSE125469_Differentially_GSE125469_Expressed_Genes.xlsx 695.3 Kb (ftp)(http) XLSX
GSE125469_Differentially_GSE125469_Expressed_circularRNA.xlsx 28.2 Kb (ftp)(http) XLSX
GSE125469_Expression_Gene.xlsx 1.9 Mb (ftp)(http) XLSX
GSE125469_Expression_circularRNA.xlsx 873.3 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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