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Series GSE125457 Query DataSets for GSE125457
Status Public on Jun 24, 2020
Title Expression data in 4T1 cells after co-culture with murine PI/AI BMDM macrophages
Organism Mus musculus
Experiment type Expression profiling by array
Summary Macrophages can exert both pro- and anti-tumorigenic features. Interaction between tumour-associated macrophages and tumour cells trigger signalling mechanisms affecting gene expression patterns in both cells. Changes in tumor gene expression affect essential physiological processes such as metabolism, proliferation or adhesion that can lead to an increased invasive and metastatic potential.
We used microarrays to detail the global program of gene expression in tumour cells after pro-inflammatory (anti-tumorigenic, M1 or PI) or anti-inflammatory (pro-tumorigenic, M2 or AI) and identified distinct classes of down-regulated genes during this process.
Overall design Murine macrophages were differentiated after bone marrow extraction (BMDM) and polarised towards a PI or AI phenotype. Macrophages were then co-cultured for 24 hours with 4T1 (triple negative breast cancer mouse cells). Both populations were separated and RNA extraction and hybridization on Affymetrix microarrays was performed on the tumour fraction. 3 biological replicates (macrophages derived from individual mice) were used in the experiment.
Contributor(s) Gomez V, Ng T, Arno M
Citation(s) 33023985
Submission date Jan 22, 2019
Last update date Oct 07, 2020
Contact name Valentí Gómez
Organization name University College London
Department UCL Cancer Institute
Lab Oncology
Street address UCL Cancer Institute, 72 Huntley Street
City London
ZIP/Postal code WC1E 6DD
Country United Kingdom
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (6)
GSM3574381 4T1 cells co-cultured with PI macrophages, biological rep1
GSM3574382 4T1 cells co-cultured with PI macrophages, biological rep2
GSM3574383 4T1 cells co-cultured with PI macrophages, biological rep3
BioProject PRJNA516428

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125457_Matrix_file.xls.gz 3.6 Mb (ftp)(http) XLS
GSE125457_RAW.tar 52.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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