|
| Status |
Public on Jan 15, 2019 |
| Title |
miRNA data from melanoma-derived exosomes and melanoma cell lines |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Identify miRNAs enriched or overexpressed in melanoma-derived exosomes compared to melanoma cells. We analyzed the 2,578 human miRNAs located in the array according to two ways. The first one identified the differentially expressed miRNAs between melanoma-derived exosomes and their parent cells. In this way, we found 198 miRNAs up-regulated in melanoma cell lines compared to their exosomes and 206 miRNAs up-regulated in exosomes compared to their donor cells. The second way of analysis identifies the most expressed miRNAs in the melanoma-derived exosomes without assumption about their expression in parent cells. We defined two criteria; the first one is a RMA (Robust Multi-Array Average, a good alternative to gene expression value) above 5, corresponding to the mean expression level of the array and a SD ≤ 0.2 between all samples to discover miRNAs expressed uniformly between melanoma-derived exosomes M113 and M117. We identified 44 miRNAs under these criteria.
|
| |
|
| Overall design |
We used microarray to identified miRNA enriched or overexpressed in melanoma-derived exosomes compared to their parent cells.
|
| |
|
| Contributor(s) |
Delphine F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 14, 2019 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Delphine Fradin |
| E-mail(s) |
delphine.fradin@inserm.fr
|
| Organization name |
CRCI2NA, Inserm UMR1307, CNRS UMR6075
|
| Street address |
8 quai Moncousu
|
| City |
Nantes |
| ZIP/Postal code |
44007 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
|
| Samples (10)
|
| GSM3561420 |
melanoma cell line M117, biological rep2 |
| GSM3561421 |
melanoma-derived exosome M113, biological rep1 |
| GSM3561422 |
melanoma cell line M113, biological rep1 |
| GSM3561423 |
melanoma-derived exosome M113, biological rep2 |
| GSM3561424 |
melanoma cell line M113, biological rep2 |
| GSM3561425 |
melanoma-derived exosome M113, biological rep3 |
| GSM3561426 |
melanoma cell line M113, biological rep3 |
| GSM3561427 |
melanoma-derived exosome M117, biological rep1 |
| GSM3561428 |
melanoma cell line M117, biological rep1 |
| GSM3561429 |
melanoma-derived exosome M117, biological rep2 |
|
| Relations |
| BioProject |
PRJNA514998 |
Less...
More...