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| Status |
Public on Oct 01, 2019 |
| Title |
Effects of Aflibercept administration at 25 mg per kg body weight in retinas of mice subjected to the oxygen-induced retinopathy protocol |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We aimed at identifying the regulatory network of Aflibercept in the context of pathological neovascularization by using the oxygen-induced retinopathy mouse model as a surrogate of proliferative diabetic retinopathy
Total RNA was collected from entire retinal cups dissected from mice subjected to the oxygen-induced retinopathy protocol and subsequently injected with Aflibercept either on postnatal day 13 (collection on day 14) or on postnatal day 13 and 15 (collection on day 17). Non-injected OIR littermates and age-matched mice housed in normoxic conditions were used as controls.
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| Overall design |
Three treatments were considered, namely normoxic controls, OIR and OIR+AFL mice. Three mice were considered per treatment, i.e. each group contained three biological replicates. Two ages were considered, namely P14 and P17. Thus, 18 samples were analyzed in total - 2 ages, 3 treatments, and 3 replicates per age and treatment. Each sample consists of total RNA isolated from the 2 entire retinas of 2 mice, i.e. 4 pooled retinas in total.
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| Contributor(s) |
Rojo Arias JE, Jászai J |
| Citation(s) |
31550048, 34321516 |
| |
| Submission date |
Jan 11, 2019 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Jesus Eduardo Rojo Arias |
| E-mail(s) |
erojoarias@gmail.com
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| Organization name |
University of Cambridge
|
| Department |
Wellcome-MRC Cambridge Stem Cell Institute
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| Lab |
Alcolea
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| Street address |
Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, Puddicombe Way
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21877 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA514455 |
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