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| Status |
Public on Feb 11, 2019 |
| Title |
CD69+ memory T lymphocytes of the bone marrow and spleen express the signature transcripts of tissue-resident memory T lymphocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Expression profiling by array
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| Summary |
To understand tissue resident features of memory CD4+ and CD8+ T lymphocytes of the bone marrow and/or spleen according to expressing or not the tissue retention marker CD69, we performed whole transcriptome profiling of ex vivo antigen-specific CD69+ and CD69- memory CD4+ T cells isolated from bone marrow and spleen, and ex vivo CD69+ and CD69- memory CD8+ T cells isolated from bone marrow.
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| Overall design |
For CD4 analysis, 8-week-old C57BL/6 mice were primed at day 0 with LCMV.GP61-80-NP-MSA + poly(I:C) and immunized again at day 14 with LCMV.GP61-80 + poly(I:C). Sixty days after the last immunization mice were sacrificed and LCMV.GP66–77-specific CD69+ and CD69- memory CD4+ T cells were isolated from BM and spleen by FACS. The whole transcriptome profiling of purified cells was performed by RNA-seq. For CD8 analysis, single cell suspensions were prepared from bone marrow of 8-week old untreated C57BL/6 male mice. CD8+ T cells were pre-enriched with anti-CD8a microbeads and the CD69+ and CD69- subpopulations of memory CD8+ T cells were then FACS purified. The whole transcriptome profiling of sorted cells was analyzed by microarray analysis.
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| Citation(s) |
30673129 |
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| Submission date |
Jan 08, 2019 |
| Last update date |
Jun 24, 2019 |
| Contact name |
Pawel Durek |
| E-mail(s) |
pawel.durek@drfz.de
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| Organization name |
Deutsches Rheuma-Forschungszentrum
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| Street address |
Charitéplatz 1
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (2) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (18)
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| This SuperSeries is composed of the following SubSeries: |
| GSE124794 |
Whole-transcriptome profiling of LCMV.GP66-77 specific CD4 T cells isolated from bone marrow (BM) and spleen, 60 days after last immunization [RNA-seq] |
| GSE124795 |
Whole-transcriptome profiling of murine steady state CD69+ and CD69- memory CD8+ T cells from the bone marrow [expression microarray] |
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| Relations |
| BioProject |
PRJNA513497 |
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