|
| Status |
Public on Jan 07, 2022 |
| Title |
Multiplex 5mC Marker Barcode Counting (MMBC-seq): A sensitive detection method for cell-free DNA methylation |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
The detection of hypermethylation markers on cell-free DNA (cfDNA) in biological fluids is a promising and non-invasive approach for early diagnosis and monitoring of human diseases. However, it is challenging to detect hypermethylation markers in a high-throughput, sensitive, and cost-effective manner. Here we presented a multiplex 5-methylcytosine marker barcode counting (MMBC-seq) technique and reported its clinical application for cfDNA from peripheral plasma samples. We identified an MMBC cancer detection panel and developed a scoring system to differentiate cancer versus healthy controls. In a multiple-cancer case-control study, the panel achieved a sensitivity and specificity of 80.2% and 95.7% respectively (AUC 0.906, 95% CI 0.846-0.948). The results suggest that MMBC-seq has great potential to realize non-invasive, flexible and clinically applicable cancer detection.
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| |
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| Overall design |
A total of 58 healthy individuals, 121 cancer patients, 19 patients with benign disease from China.
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| |
|
| Contributor(s) |
He C, Nie J |
| Citation missing |
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| |
| Submission date |
Jan 07, 2019 |
| Last update date |
Jan 07, 2022 |
| Contact name |
JI NIE |
| E-mail(s) |
jinie@uchicago.edu
|
| Organization name |
The University of Chicago
|
| Department |
Chemistry
|
| Street address |
929E 57th
|
| City |
CHICAGO |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (198)
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| Relations |
| BioProject |
PRJNA513381 |
| SRA |
SRP176482 |
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