|
| Status |
Public on Oct 24, 2019 |
| Title |
Single cell multi-omics analysis reveals novel roles for DNA methylation in sensory neuron injury responses |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
We used single cell multi-omics method to simultaneously profiled both DNA methylome and mRNA transcriptome from single DRG sensory neurons under either control or peripheral nerve injury condition.
|
| |
|
| Overall design |
In this study, we performed sciatic nerve injury surgery on C57BL/6 and CAST/Ei crossing mice at different ages, and performed scMT-seq, μRNA-seq (low-input RNA-seq) and μWGBS (low-input WGBS) on sensory neurons under intact and injured condition. Bioinformatic analysis revealed novel role for DNA methylation in sensory neuron injury responses.
|
| |
|
| Contributor(s) |
An Q, Hu Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 07, 2019 |
| Last update date |
Oct 26, 2019 |
| Contact name |
Qin An |
| E-mail(s) |
anqin314@gmail.com
|
| Organization name |
ucla
|
| Department |
Department of Human Genetics
|
| Lab |
Guoping Fan Lab
|
| Street address |
Room 6554 Gonda Building, UCLA
|
| City |
los angeles |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
90024 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (230)
|
|
| Relations |
| BioProject |
PRJNA513289 |
| SRA |
SRP176417 |
Less...
More...