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| Status |
Public on Jul 01, 2019 |
| Title |
mRNA expression profile of mouse CD4 T cells (treated vs. untreated with PM2.5 suspension) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: PM2.5 can impact mouse TH17 cell differentitation. To Investigator the genes altered by PM2.5 during TH17 differentiation, mRNA-seq was performed.
Methods: Naïve T cells were isolated from mouse spleen by magnetic beads. These naïve T cells were stimulated by CD3/CD28 antibodies and cultured under TH17 polarization conditions for 4 days before harvested. For PM2.5 group, 100ug/ml of PM2.5 suspensions were added to culture medium 6 hours after stimulation. The total RNA was extracted using the TRIzol reagent (Invitrogen). The library construction and sequencing was performed by Illumina Xten at Shanghai Biotechnology Corporation.
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| Overall design |
PM2.5 treated group was compared to control group. Each group has 3 samples.
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| Contributor(s) |
Zhou Y, Sun L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 04, 2019 |
| Last update date |
Jul 01, 2019 |
| Contact name |
Sun Licheng |
| E-mail(s) |
16111510037@fudan.edu.cn
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| Organization name |
Fudan University
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| Street address |
No. 399, wanyuan road
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| City |
Shanghai |
| ZIP/Postal code |
201102 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA513007 |
| SRA |
SRP175258 |
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