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Series GSE124557 Query DataSets for GSE124557
Status Public on Apr 30, 2019
Title CUT&Tag for efficient epigenomic profiling of small samples and single cells
Organism Homo sapiens
Experiment type Other
Genome binding/occupancy profiling by high throughput sequencing
Summary Many chromatin features play critical roles in regulating gene expression. A complete understanding of gene regulation will require the mapping of specific chromatin features in small samples of cells at high resolution. Here we describe Cleavage Under Targets and Tagmentation (CUT&Tag), an enzyme-tethering strategy that provides efficient high-resolution sequencing libraries for profiling diverse chromatin components. In CUT&Tag, a chromatin protein is bound in situ by a specific antibody, which then tethers a proteinA-Tn5 transposase fusion protein. Activation of the transposase efficiently generates fragment libraries with high resolution and exceptionally low background. All steps from live cells to sequencing-ready libraries can be performed in a single tube on the benchtop or a microwell in a high-throughput pipeline, and the entire procedure can be performed in one day. We demonstrate the utility of CUT&Tag by profiling histone modifications, RNA Polymerase II and transcription factors on low cell numbers and single cells.
 
Overall design CUT&Tag followed by Illumina sequencing
 
Contributor(s) Kaya-Okur HS, Wu SJ, Pledger ES, Ahmad K, Henikoff S
Citation(s) 31036827
Submission date Jan 02, 2019
Last update date May 09, 2019
Contact name Jorja Henikoff
E-mail(s) jorja@fhcrc.org
Phone 206-667-4850
Organization name Fred Hutchinson Cancer Research Center
Department Basic Sciences
Lab Henikoff
Street address 1100 Fairview AV N, A1-162
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (59)
GSM3536497 H1_K27ac_(20180919_HK_Hs_H1I_K27ac_0912)
GSM3536498 H1_K27me3_(20180919_HK_Hs_H1I_K27me3_0912)
GSM3536499 H1_K4me1_(20180919_HK_Hs_H1I_K4me1_0912)
This SubSeries is part of SuperSeries:
GSE124690 CUT&Tag for efficient epigenomic profiling of small samples and single cells.
Relations
BioProject PRJNA512492
SRA SRP175021

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124557_Bench_top_CnT.txt.gz 1.4 Kb (ftp)(http) TXT
GSE124557_Bench_top_CnT_v2.txt.gz 1.6 Kb (ftp)(http) TXT
GSE124557_RAW.tar 2.5 Gb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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