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Series GSE124395 Query DataSets for GSE124395
Status Public on Jun 18, 2019
Title A Human Liver Cell Atlas reveals Heterogeneity and Epithelial Progenitors
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We perfomed single-cell RNA-sequnecing of around 10,000 cells from normal human liver tissue to construct a human liver cell atlas. We reveal previously unknown subtypes in different cell type compartments. We also use our normal liver cell atlas to infer perturbed phenoytpes of cells from HCC samples, human cells engrafted into a mouse liver and liver organoids.
 
Overall design Single cells were isolated from human liver resection specimens and then sorted by FACS into 384 well plates in a unbiased way and on the basis of cell surface markers for distinct cell types. ScRNA-seq was done using the mCelSeq2 protocol
cellbarcodes_cellid.csv Supplemetary file contains cellds and one of the 192 unique cellbarcode associated with the cellid.
 
Contributor(s) Aizarani N, Grün D
Citation(s) 31292543
Submission date Dec 26, 2018
Last update date Apr 20, 2022
Contact name Nadim Aizarani
E-mail(s) aizarani@ie-freiburg.mpg.de
Organization name MPI-IE
Department Independent group leaders
Lab Gruen
Street address Stübeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (319)
GSM3531672 P301_3
GSM3531673 P301_4
GSM3531674 P301_5
Relations
BioProject PRJNA511895
SRA SRP174502

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124395_File_Descriptions.txt.gz 305 b (ftp)(http) TXT
GSE124395_Normalhumanlivercellatlasdata.txt.gz 26.8 Mb (ftp)(http) TXT
GSE124395_Normalhumanliverdata.RData.gz 42.7 Mb (ftp)(http) RDATA
GSE124395_RAW.tar 87.5 Mb (http)(custom) TAR (of CSV)
GSE124395_celseq_barcodes.192.txt.gz 910 b (ftp)(http) TXT
GSE124395_clusterpartition.txt.gz 32.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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