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| Status |
Public on Jun 30, 2020 |
| Title |
Human Bacille Calmette-Guérin vaccination elicits trained immunity via the hematopoietic progenitor compartment [bone marrow] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Induction of trained immunity by human Bacille-Calmette-Guérin (BCG) vaccination is implicated in the beneficial heterologous effects of the vaccine, but the underlying mechanisms remain elusive. We performed global transcriptome analysis of sorted progenitors from bone marrow before (D0) and 90 days after vaccination (D90). BCG vaccination induced transcriptomic myeloid priming of the hematopoietic stem and progenitor cell (HSPC) compartment marked by the upregulation of myeloid and granulocytic pathways alongside the induction of transcription factors connected to myeloid cell function, namely Hepatocyte Nuclear Factors (HNF). These findings are corroborated by higher granulocyte numbers in BCG-vaccinated infants, HNF1-related SNP variants correlating with immune training and elevated serum levels of the HNF1 target gene SERPINA1. Taken together, we reveal a transcriptomic reprograming of HSPCs and peripheral monocytes as a trait of in vivo BCG-induced trained immunity.
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| Overall design |
mRNA profiles of sorted hematopoietic stem and progenitor cells (HSPC, i.e. pooled HSC & MPP) from human bone marrow. Sample collection was performed before and 90 days after vaccination from the same individuals (n=15 for BCG-vaccinated individuals, n=5 for placebo-treated individuals) resulting in a total of 39 transcriptomic profiles (one sample (Donor 9 D0) was identified as outlier and excluded).
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| Contributor(s) |
Cirovic B, de Bree CL, Picelli S, Händler K, Klee K, Schultze JL, Netea MG, Schlitzer A |
| Citation(s) |
32544459 |
| |
| Submission date |
Dec 20, 2018 |
| Last update date |
May 31, 2021 |
| Contact name |
Branko Cirovic |
| Organization name |
LIMES
|
| Lab |
Myeloid Cell Biology
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| Street address |
Carl-Troll Str 31
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| City |
Bonn |
| ZIP/Postal code |
53111 |
| Country |
Germany |
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| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (39)
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| This SubSeries is part of SuperSeries: |
| GSE124220 |
Human Bacille Calmette-Guérin vaccination elicits trained immunity via the hematopoietic progenitor compartment |
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| Relations |
| BioProject |
PRJNA511004 |
| SRA |
SRP174103 |
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