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| Status |
Public on Sep 18, 2019 |
| Title |
Transcriptome of CRPV-induced wart tissues |
| Organism |
Oryctolagus cuniculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cottontail rabbit papillomavirus (CRPV) infection in susceptible animals represents an important model for studying papillomavirus pathogenesis and tumorigenesis. The goal of this project is to determine a comprehensive transcriptome of CRPV-induced wart tissues generated by different routes of infection.
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| Overall design |
The New Zealand rabbits with prewounded back skin were infected with CRPV Hershey strain via two routes of infection: a) cutaneuosly by scratching the pre-wounded back skin; b) intravenous injection into ear vein. The medium-size tumors occurred at the pre-wounded back skin area were harvested. The total RNA was isolated using TRIpure Reagent (Roche) and DNase-treated on column using RNeasy kit (Qiagen). Total stranded RNA-seq was performed on Ribo-minus RNA using True-seq kit with 125 bp modality and 100 million reads depth.
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| Contributor(s) |
Zheng Z, Majerciak V, Jiang P, Meyer TJ |
| Citation(s) |
31340720 |
| |
| Submission date |
Dec 20, 2018 |
| Last update date |
Sep 18, 2019 |
| Contact name |
Thomas Joshua Meyer |
| E-mail(s) |
thomas.meyer@nih.gov
|
| Organization name |
National Institutes of Health
|
| Department |
National Cancer Institute
|
| Lab |
CCBR
|
| Street address |
41 Center Drive, Building 41, Room B620
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL25392 |
Illumina HiSeq 4000 (Oryctolagus cuniculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA510994 |
| SRA |
SRP174093 |
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