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| Status |
Public on Jul 08, 2019 |
| Title |
Genome-wide identification of 5hmCpGs in multiple myeloma |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
We report the genomic localization of 5-hydroxymethylcytosines in multiple myeloma patients through the use of selective chemical labeling (SCL) and exonuclease digestion. Data highlighted super-enhancers and enhancers specifically undergoing active DNA demethylation in multiple myeloma cells.
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| Overall design |
Genomic DNA from multiple myeloma (MM) cells was extracted and submitted to the SCL-exo protocol. After sequencing with a HiSeq 1500 (Illumina), reads were mapped to hg19 and processed to generate a single-CpG signal file (wig file) indicating the relative levels of hydroxymethylation.
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| |
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| Contributor(s) |
Salbert G, Avner S |
| Citation(s) |
31221779 |
| |
| Submission date |
Dec 20, 2018 |
| Last update date |
Jul 08, 2019 |
| Contact name |
Gilles Salbert |
| Organization name |
University Rennes 1
|
| Department |
IGDR UMR6290
|
| Street address |
263 Av. Gal. Leclerc
|
| City |
Rennes |
| ZIP/Postal code |
35200 |
| Country |
France |
| |
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| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA510971 |
| SRA |
SRP174036 |
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