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Series GSE124080 Query DataSets for GSE124080
Status Public on Dec 19, 2018
Title Primary term human cytotrophoblast, untreated [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chromatin alterations are important mediators of gene expression changes. We have recently shown that activated non-canonical NF-κB signaling (RelB/p52) recruits histone acetyltransferase CBP and deacetylase HDAC1 to selectively acetylate H3K9 (H3K9ac) to induce expression of corticotropin-releasing hormone (CRH) and prostaglandin-endoperoxide synthase-2 (PTGS2) in the human placenta. Both of these genes play a role in initiating. We performed chromatin immunoprecipitation followed by gene sequencing (ChIP-seq) in primary term human cytotrophoblast (CTB) with use of antibodies to RelB, CBP, HDAC1 and H3K9ac. We further associated these chromatin alterations with gene expression changes from mid-trimester to term in CTB by RNA sequencing (RNA-seq). We detected a genome-wide differential gene enrichment between mid-trimester and term human placenta. Pathway analysis identified that cytokine-cytokine receptor interaction, NF-κB, and TNF are the leading pathways enriched in term placenta and associated with these chromatin alterations. Our analysis has provided the first-time characterization of the key players of human placental origin with molecular changes resulting from chromatin modifications, which could drive human labor.
Overall design 3 individual placental specimens from full-term pregnancies were collected from healthy women with estimated gestational age (EGA) of 38 and 40 weeks who were delivered by Cesarean section (C-section). 3 individual mid-trimester placentas were collected from healthy individuals with EGA between18 to 22 weeks following elective termination of pregnancy.
Contributor(s) Wang B
Citation(s) 30642670
Submission date Dec 18, 2018
Last update date Mar 27, 2019
Contact name Bingbing Wang
Organization name Rutgers RWJMS
Street address 125 Paterson St Room 2143
City New Brunswick
State/province New Jersey
ZIP/Postal code 08901
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (12)
GSM3520243 IPed by CBP antibody in human term CTB-Rep1
GSM3520244 IPed by CBP antibody in human term CTB-Rep2
GSM3520245 IPed by CBP antibody in human term CTB-Rep3
BioProject PRJNA510668
SRA SRP173845

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Supplementary file Size Download File type/resource
GSE124080_RAW.tar 9.6 Mb (http)(custom) TAR (of XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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