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Series GSE124080 Query DataSets for GSE124080
Status Public on Dec 19, 2018
Title Primary term human cytotrophoblast, untreated [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chromatin alterations are important mediators of gene expression changes. We have recently shown that activated non-canonical NF-κB signaling (RelB/p52) recruits histone acetyltransferase CBP and deacetylase HDAC1 to selectively acetylate H3K9 (H3K9ac) to induce expression of corticotropin-releasing hormone (CRH) and prostaglandin-endoperoxide synthase-2 (PTGS2) in the human placenta. Both of these genes play a role in initiating. We performed chromatin immunoprecipitation followed by gene sequencing (ChIP-seq) in primary term human cytotrophoblast (CTB) with use of antibodies to RelB, CBP, HDAC1 and H3K9ac. We further associated these chromatin alterations with gene expression changes from mid-trimester to term in CTB by RNA sequencing (RNA-seq). We detected a genome-wide differential gene enrichment between mid-trimester and term human placenta. Pathway analysis identified that cytokine-cytokine receptor interaction, NF-κB, and TNF are the leading pathways enriched in term placenta and associated with these chromatin alterations. Our analysis has provided the first-time characterization of the key players of human placental origin with molecular changes resulting from chromatin modifications, which could drive human labor.
 
Overall design 3 individual placental specimens from full-term pregnancies were collected from healthy women with estimated gestational age (EGA) of 38 and 40 weeks who were delivered by Cesarean section (C-section). 3 individual mid-trimester placentas were collected from healthy individuals with EGA between18 to 22 weeks following elective termination of pregnancy.
 
Contributor(s) Wang B
Citation(s) 30642670
Submission date Dec 18, 2018
Last update date Mar 27, 2019
Contact name Bingbing Wang
E-mail(s) wangbi@rwjms.rutgers.edu
Organization name Rutgers RWJMS
Street address 125 Paterson St Room 2143
City New Brunswick
State/province New Jersey
ZIP/Postal code 08901
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (12)
GSM3520243 IPed by CBP antibody in human term CTB-Rep1
GSM3520244 IPed by CBP antibody in human term CTB-Rep2
GSM3520245 IPed by CBP antibody in human term CTB-Rep3
Relations
BioProject PRJNA510668
SRA SRP173845

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Supplementary file Size Download File type/resource
GSE124080_RAW.tar 9.6 Mb (http)(custom) TAR (of XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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