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Series GSE124054 Query DataSets for GSE124054
Status Public on Jun 05, 2019
Title KLRG1 and NKp46 expressing CD117+ human innate lymphoid cell precursors are biased to ILC2 and ILC3, respectively
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Recently, circulating multi- and uni-potent human ILC precursors (ILCP) have been found in a lymphocyte population that expresses CD117 and CD127 but lacks CRTH2 and NKp44. However, these ILCPs have not been extensively characterized. We performed an unbiased Hierarchical Stochastic Neighbor Embedding (HSNE) analysis of the phenotype of peripheral blood CD117+ ILCPs which revealed the presence of three major subsets: the first expressed NKp46, the second expressed both NKp46 and CD56, while the third expressed KLRG1. Analysis of their differentiation potential on bulk and clonal levels, cytokine production and the transcriptome revealed that the NKp46+ ILCPs contain high frequencies of ILC3 precursors whereas a minority can differentiate into ILC1/NK-like cells. In contrast, KLRG1+ ILCPs are biased to the ILC2 lineage, but are highly plastic and epigenetically primed to differentiate into other ILC subsets depending on the activation signals they receive.
 
Overall design biological replicate (n=3) ATAC-Seq experiments
 
Contributor(s) Stadhouders R, Hendriks RW, Bal SM
Citation(s) 31201208
Submission date Dec 18, 2018
Last update date Aug 20, 2019
Contact name Ralph Stadhouders
E-mail(s) stadhoudersralph@gmail.com
Organization name Erasmus Medical Center
Department Pulmonary Medicine
Street address Dr. Molewaterplein 50
City Rotterdam
ZIP/Postal code 3015 GE
Country Netherlands
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (6)
GSM3519811 atac_ILC2_rep1
GSM3519812 atac_ILC2_rep2
GSM3519813 atac_ILC2_rep3
Relations
BioProject PRJNA510626
SRA SRP173816

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124054_RAW.tar 4.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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