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Series GSE12403 Query DataSets for GSE12403
Status Public on Aug 12, 2008
Title Expression data from Arabidopsis seed compartments at the linear-cotyledon stage
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary We collected linear-cotyledon stage seed compartments from 5 to 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing linear-coyledon-stage embryos. For the purposes of this study we captured 7 compartments: embyro proper, cellularized endosperm, chalazal endosperm, chalazal seed coat, general seed coat, whole seeds and micropylar endosperm.
 
Overall design linear-cotyledon stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, cellularized endosperm, general seed coat, chalazal seed coat, whole seeds and micropylar endosperm, 2 biological replicates each).
 
Contributor(s) Stone SL, Pelletier J, Kirkbride RC, Lu XH, Belmonte MF, Fei J, Cheng C, Golberg RB, Harada JJ
Citation(s) 23319655, 20385809
Submission date Aug 11, 2008
Last update date Aug 28, 2018
Contact name John J Harada
E-mail(s) jjharada@ucdavis.edu
Phone 530-752-0673
Organization name University of California, Davis
Department Plant Biology
Lab Harada
Street address One Shields Avenue
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (14)
GSM311287 Laser capture microdissected (LCM) embryo proper at the linear-cotyledon stage, biological replicate 1
GSM311288 Laser capture microdissected (LCM) embryo proper at the linear-cotyledon stage, biological replicate 2
GSM311289 Laser capture microdissected (LCM) cellularized endosperm at the linear-cotyledon stage, biological replicate 1
This SubSeries is part of SuperSeries:
GSE12404 Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development
Relations
BioProject PRJNA114187

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12403_RAW.tar 27.1 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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