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| Status |
Public on Aug 12, 2008 |
| Title |
Expression data from Arabidopsis seed compartments at the linear-cotyledon stage |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We collected linear-cotyledon stage seed compartments from 5 to 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing linear-coyledon-stage embryos. For the purposes of this study we captured 7 compartments: embyro proper, cellularized endosperm, chalazal endosperm, chalazal seed coat, general seed coat, whole seeds and micropylar endosperm.
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| Overall design |
linear-cotyledon stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, cellularized endosperm, general seed coat, chalazal seed coat, whole seeds and micropylar endosperm, 2 biological replicates each).
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| Contributor(s) |
Stone SL, Pelletier J, Kirkbride RC, Lu XH, Belmonte MF, Fei J, Cheng C, Golberg RB, Harada JJ |
| Citation(s) |
23319655, 20385809 |
| Submission date |
Aug 11, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
John J Harada |
| E-mail(s) |
jjharada@ucdavis.edu
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| Phone |
530-752-0673
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| Organization name |
University of California, Davis
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| Department |
Plant Biology
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| Lab |
Harada
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| Street address |
One Shields Avenue
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| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (14)
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| GSM311287 |
Laser capture microdissected (LCM) embryo proper at the linear-cotyledon stage, biological replicate 1 |
| GSM311288 |
Laser capture microdissected (LCM) embryo proper at the linear-cotyledon stage, biological replicate 2 |
| GSM311289 |
Laser capture microdissected (LCM) cellularized endosperm at the linear-cotyledon stage, biological replicate 1 |
| GSM311290 |
Laser capture microdissected (LCM) cellularized endosperm at the linear-cotyledon stage, biological replicate 2 |
| GSM311291 |
Laser capture microdissected (LCM) chalazal endosperm at the linear-cotyledon stage, biological replicate 1 |
| GSM311292 |
Laser capture microdissected (LCM) chalazal endosperm at the linear-cotyledon stage, biological replicate 2 |
| GSM311293 |
Laser capture microdissected (LCM) chalazal seed coat at the linear-cotyledon stage, biological replicate 1 |
| GSM311294 |
Laser capture microdissected (LCM) chalazal seed coat at the linear-cotyledon stage, biological replicate 2 |
| GSM311295 |
Laser capture microdissected (LCM) general seed coat at the linear-cotyledon stage, biological replicate 1 |
| GSM311296 |
Laser capture microdissected (LCM) general seed coat at the linear-cotyledon stage, biological replicate 2 |
| GSM499418 |
Laser capture microdissected (LCM) whole seeds at the linear-cotyledon stage, biological replicate 1 |
| GSM499419 |
Laser capture microdissected (LCM) whole seeds at the linear-cotyledon stage, biological replicate 2 |
| GSM499420 |
Laser capture microdissected (LCM) micropylar endosperm at the linear-cotyledon stage, biological replicate 1 |
| GSM499421 |
Laser capture microdissected (LCM) micropylar endosperm at the linear-cotyledon stage, biological replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE12404 |
Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development |
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| Relations |
| BioProject |
PRJNA114187 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12403_RAW.tar |
27.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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