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Series GSE12402 Query DataSets for GSE12402
Status Public on Aug 12, 2008
Title Expression data from Arabidopsis seed compartments at the pre-globular stage
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary We collected pre-globular stage seed compartments from 7-micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments of seeds containing pre-globular-stage embryos was identified as those seeds containing embryo propers made up of between 2 and 8 cells. For the purposes of this study we captured 6 compartments: embyro proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, chalazal seed coat and general seed coat. Serial sections of entire seeds were also captured for comparison.

Keywords: cell type comparison
 
Overall design Pre-globular stage seed compartments or whole seeds were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, general seed coat, chalazal seed coat, whole seeds, 2 biological replicates each).
 
Contributor(s) Stone SL, Pelletier J, Kirkbride RC, Lu XH, Belmonte MF, Fei J, Cheng C, Golberg RB, Harada JJ
Citation(s) 23319655, 20385809
Submission date Aug 11, 2008
Last update date Aug 28, 2018
Contact name John J Harada
E-mail(s) jjharada@ucdavis.edu
Phone 530-752-0673
Organization name University of California, Davis
Department Plant Biology
Lab Harada
Street address One Shields Avenue
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (14)
GSM311273 Laser capture microdissected (LCM) embryo proper at the pre-globular stage, biological replicate 1
GSM311274 Laser capture microdissected (LCM) embryo proper at the pre-globular stage, biological replicate 2
GSM311275 Laser capture microdissected (LCM) micropylar endosperm at the pre-globular stage, biological replicate 1
This SubSeries is part of SuperSeries:
GSE12404 Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development
Relations
BioProject PRJNA114185

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12402_RAW.tar 27.1 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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