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Series GSE123817 Query DataSets for GSE123817
Status Public on Jun 05, 2019
Title KLRG1 and NKp46 discriminate subpopulations of human CD117+ CRTH2- ILCs biased toward ILC2 or ILC3
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Recently, circulating multi- and uni-potential human ILC precursors (ILCP) have been found in a lymphocyte population that expresses CD117 and CD127 but lack CRTH2 and NKp44. However, these ILCPs have not been extensively characterized. We performed an unbiased Hierarchical Stochastic Neighbor Embedding (HSNE) analysis of the phenotype of peripheral blood CD117+ ILCPs which revealed the presence of three major subsets; the first expressed NKp46, the second expressed both NKp46 and CD56 and the third expressed KLRG1. Analysis of the differentiation potential of these cells on bulk and clonal levels, cytokine production and the transcriptome revealed that the NKp46+ ILCPs contain high frequencies of ILC3 precursors whereas a minority can differentiate into ILC1/NK-like cells. In contrast KLRG1+ ILCPs are biased to the ILC2 lineage, but are highly plastic and epigenetically poised to differentiate to other ILC subsets depending on the activation signals they receive.
 
Overall design 24 samples of 5 different cell types 6 replicates for 3 cell types and 3 replicates for 2 cell types
 
Contributor(s) Nagasawa M, Heesters BA, Stadhouders R, Kradolfer CM, Krabbendam L, Golebski K, Hendriks RW, Spits H, Bal SM
Citation(s) 31201208
Submission date Dec 13, 2018
Last update date Aug 20, 2019
Contact name Balthasar Adrianus Heesters
Organization name AMC
Department EXIM
Lab Spits
Street address Meibergdreeg 9
City Amsterdam
State/province Noord-Holland
ZIP/Postal code 1105 AZ
Country Netherlands
 
Platforms (1)
GPL24324 [Clariom_S_Human_HT] Affymetrix Clariom S Assay HT, Human (Includes Pico Assay)
Samples (24)
GSM3511834 T44negILC3 donor: 8
GSM3511835 T44negILC3 donor: 3
GSM3511836 T44negILC3 donor: 11
Relations
BioProject PRJNA509919

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE123817_RAW.tar 24.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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