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| Status |
Public on Aug 20, 2019 |
| Title |
ROLE OF EXOSOMAL miRNAs IN METASTATIC POST-TRANSPLANT COLORECTAL CARCINOMA UNDER RAPAMYCIN AND CYCLOSPORINE (miRNA-4) |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Immunosuppression plays a crucial role in the development of cancer which remains a major cause of mortality in kidney transplant recipients. Cancer Exosomes (Exos) are extracellular vesicles described as modulators of tumor invasion and metastasis. This paper describes the effect of RAPA and CsA, two immunosuppressive drugs with different oncogenic proprieties, in Exos of colorectal cancer (CRC) cell lines. RAPA induces an increased Exos production and an overexpression of miR-6127, miR-6746-5p, and miR-6787-5p in Exos from a metastatic cell line. These miRNAs produce a significant down-regulation of epigenetic genes involved in cell cycle, chromatin and DNA regulation pre-metastatic niche. Our results describe a potential mechanism by RAPA in modulating pre-metastatic niche in post-transplant metastatic CRC through these exosomal miRNAs.
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| Overall design |
HCT166 and SW480 cell lines were treated with Rapamycin and cyclosporine using two replicates.Untreated cell lines were also included.
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| |
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| Contributor(s) |
Tubita V, Ramirez-Bajo MJ, Diekmann F, Revuelta I |
| Citation(s) |
31371743 |
| |
| Submission date |
Dec 12, 2018 |
| Last update date |
Aug 20, 2019 |
| Contact name |
Juanjo Lozano |
| E-mail(s) |
juanjo.lozano@ciberehd.org
|
| Organization name |
CIBEREHD
|
| Department |
Plataforma de Bioinformatica
|
| Street address |
C/ Rosselló 153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
|
| Samples (12)
|
| GSM3509917 |
HRA 1: HCT116 cell line treated with Rapamycin [1] |
| GSM3509918 |
HRA 2: HCT116 cell line treated with Rapamycin [2] |
| GSM3509919 |
HUT 1: HCT116 cell line untreated [1] |
| GSM3509920 |
HUT 2: HCT116 cell line untreated [2] |
| GSM3509921 |
480CSA1: SW480 cell line treated with Cyclosporine [1] |
| GSM3509922 |
480CSA2: SW480 cell line treated with Cyclosporine [2] |
| GSM3509923 |
480RA1: SW480 cell line treated with Rapamycin [1] |
| GSM3509924 |
480RA2: SW480 cell line treated with Rapamycin [2] |
| GSM3509925 |
480UT1: SW480 cell line untreated [1] |
| GSM3509926 |
480UT2: SW480 cell line untreated [2] |
| GSM3509927 |
HCSA1: HCT116 cell line treated with Cyclosporine [1] |
| GSM3509928 |
HCSA2: HCT116 cell line treated with Cyclosporine [2] |
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| This SubSeries is part of SuperSeries: |
| GSE123710 |
ROLE OF EXOSOMAL miRNAs IN METASTATIC POST-TRANSPLANT COLORECTAL CARCINOMA UNDER RAPAMYCIN AND CYCLOSPORINE |
|
| Relations |
| BioProject |
PRJNA509623 |
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