NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE123312 Query DataSets for GSE123312
Status Public on May 08, 2019
Title SR9009 has REV-ERB-independent effects on cell proliferation and metabolism
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The nuclear receptors (NRs) REV-ERBα and β, encoded by Nr1d1 and Nr1d2, link circadian rhythms and metabolism. REV-ERB lacks the canonical NR activation domain, and thus functions as a transcriptional repressor. Like other NRs, REV-ERBs can be regulated by ligands, including naturally occurring heme, which potentiate their repressive activity. Attempts to pharmacologically target REV-ERBs by the use of putatively specific synthetic agonists, particularly SR90096, have suggested a wide range of beneficial effects in healthy as well as diseased animal models and cell systems. For instance, Sulli et al. recently reported that REV-ERB activation by SR9009 is specifically lethal to cancer (stem) cells but not other cell types. Because REV-ERBs are core components of the molecular clock, the results were interpreted as a link between the body’s circadian timekeeping system and cancer. Moreover, increased energy expenditure after SR9009 administration decreases obesity in mice, and the reported activity of SR9009 as an exercise mimetic in skeletal muscle have resulted in online sales of the compound as a performance-enhancing drug, with advertisements reassuringly highlighting REV-ERBs as the molecular target (https://www.evolutionary.org/stenabolic-sr9009-review; https://www.simplyanabolics.com/sarms/sr9009-stenabolic/).
 
Overall design Mouse hepatocytes were derived from 10-week-old male REV-ERBab double floxed animals two weeks after AAV8-TBG-Cre-GFP (vs AAV8-TBG-GFP as a control) injection. Hepatocytes were treated with DMSO vs SR9009 for 8 hours.
 
Contributor(s) Dierickx P, Jiang C
Citation(s) 31127047
Submission date Dec 04, 2018
Last update date Aug 07, 2019
Contact name Chunjie Jiang
E-mail(s) chunjie.jiang@pennmedicine.upenn.edu
Phone 2153164211
Organization name University of Pennsylvania
Department Institute for Diabetes, Obesity, and Metabolism
Lab Mitchell A. Lazar
Street address 3400 Civic Center Boulevard
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM3500549 Hepatocytes_Control_DMSO_Rep1
GSM3500550 Hepatocytes_Control_DMSO_Rep2
GSM3500551 Hepatocytes_Control_DMSO_Rep3
Relations
BioProject PRJNA508300
SRA SRP172447

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE123312_ExpressionProfile.txt.gz 1.6 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap