|
| Status |
Public on May 02, 2019 |
| Title |
Switching from Random to Imprinted X-inactivation by Maternal PRC2 |
| Organism |
Mus musculus domesticus x M. m. molossinus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Imprinted X-inactivation is a paradigm of transgenerational epigenetic inheritance in mammals and silences genes exclusively on the paternally-inherited X-chromosome. Here, we test the hypothesis that oocyte-derived maternal Polycomb repressive complex 2 (PRC2) protein EED orchestrates the selective inactivation of the paternal-X in mouse embryos. In maternal Eed null (Eedm-/-) but not zygotic Eed null female and male embryos, the maternal X-chromosome ectopically induced Xist lncRNA, which silences X-linked genes. Subsequently, Xist was silenced from one of the two X-chromosomes in females and from the sole maternal-X in males. As a result, later-stage female Eedm-/- embryos displayed random X-inactivation. The kinetics of Xist RNA induction in Eedm-/- embryos resemble that of early human embryos, which lack oocyte-derived PRC2 and only undergo random X-inactivation. Thus, expression of PRC2 genes in the oocyte and transmission of the gene products to the embryo may dictate the occurrence of imprinted X-inactivation in mammals.
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| Overall design |
Allele-specific mRNA profile of Eedfl/fl, Eedfl/-, Eed-/-, Eedm-/-, and Eedmz-/- mouse E3.5 embryos by RNA-Seq
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| Contributor(s) |
Harris C, Cloutier M, Trotter M, Hinten M, Gayen S, Kalantry S |
| Citation(s) |
30938678 |
| |
| Submission date |
Nov 30, 2018 |
| Last update date |
Jun 18, 2019 |
| Contact name |
Sundeep Kalantry |
| E-mail(s) |
kalantry@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Human Genetics
|
| Lab |
Kalantry
|
| Street address |
1241 Catherine Street
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL16355 |
Illumina HiSeq 2000 (Mus musculus domesticus x M. m. molossinus) |
| GPL25870 |
Illumina HiSeq 4000 (Mus musculus domesticus x M. m. molossinus) |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA507766 |
| SRA |
SRP171629 |
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