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| Status |
Public on Jun 06, 2019 |
| Title |
Pioneer and repressive functions of p63 during embryonic ectoderm specification [RNA-seq] |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The transcription factor p63 is a master regulator of ectoderm development essential for epidermal specification. Although previous studies have highlighted the role of p63 triggering the epidermal transcriptomic program, its precise mechanism of target gene regulation in the complex context of a developing embryo remains poorly understood. Here, we used zebrafish embryos to analyze in vivo how p63 regulates the expression of its target genes during development. We generated tp63-knock-out mutants that recapitulate human phenotypes and show down-regulated epidermal gene expression. Following p63-binding dynamics during development, we found two distinct functions clearly separated in space and time. During early development, p63 binds enhancers associated to neural genes, where it limits Sox3 binding and reduces the expression of these neural genes. Indeed, we show that p63 and Sox3 are co-expressed in the neural plate border. Later in development, p63 binds enhancers associated to epidermal genes and promotes their expression, acting as a pioneer factor, as it binds to non-accessible chromatin and is required for its opening. Therefore, our results suggest that p63 is an important regulator of cell fate decisions during ectoderm specification, promoting the epidermal fate and inhibiting the neural program.
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| Overall design |
RNA-seq assays in tp63 zebrafish mutants
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| |
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| Contributor(s) |
Santos-Pereira JM, Gallardo-Fuentes L, Neto A, Acemel RD, Tena JJ |
| Citation(s) |
31296872 |
| |
| Submission date |
Nov 28, 2018 |
| Last update date |
Jul 26, 2019 |
| Contact name |
Juan J. Tena |
| E-mail(s) |
jjtenagu@upo.es
|
| Organization name |
CABD/CSIC
|
| Street address |
Univ. Pablo Olavide, Ctra. Utrera km1
|
| City |
Sevilla |
| ZIP/Postal code |
41013 |
| Country |
Spain |
| |
|
| Platforms (1) |
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| Samples (6)
|
| GSM3494524 |
RNAseq in zebrafish tp63 mutant embryos at 36hpf replicate 1 |
| GSM3494525 |
RNAseq in zebrafish tp63 mutant embryos at 36hpf replicate 2 |
| GSM3494526 |
RNAseq in zebrafish tp63 mutant embryos at 36hpf replicate 3 |
| GSM3494527 |
RNAseq in zebrafish wild type embryos at 36hpf replicate 1 |
| GSM3494528 |
RNAseq in zebrafish wild type embryos at 36hpf replicate 2 |
| GSM3494529 |
RNAseq in zebrafish wild type embryos at 36hpf replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE123059 |
Pioneer and repressive functions of p63 during embryonic ectoderm specification |
|
| Relations |
| BioProject |
PRJNA507426 |
| SRA |
SRP171045 |
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