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Series GSE122930 Query DataSets for GSE122930
Status Public on Oct 12, 2019
Title Single cell sequencing of mouse heart immune infiltrate in pressure overload-driven heart failure reveals extent of immune activation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Single-cell RNA sequencing is revolutionizing the mapping of tissues in physiology and pathology. Whilst immune responses are emerging as key mediators of heart failure, no thorough immune mapping of the ailing heart has been performed so far. Here we examined, at single-cell level, the cardiac immune composition after transverse aortic constriction, the standard murine model of non-ischemic heart failure. We identified the presence of most major immune cell subpopulations in the myocardium of both healthy and cardiopathic mice. Despite the absence of infectious agents or an autoimmune trigger, the ailing heart, especially at late-stage disease, is characterized by an immune activation that is both drastic and far more wide-ranging in the immune cell types involved than previously thought. The molecular-level detail we uncover in the cells undergoing activation in response to cardiac stress may enable the identification of novel biomarkers and therapeutic targets for heart failure.
 
Overall design 4 experimental condtions, two time points
 
Contributor(s) Kunderfranco P, Kallikourdis M, Condorelli G
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Submission date Nov 26, 2018
Last update date Oct 15, 2019
Contact name Paolo Kunderfranco
E-mail(s) Paolo.Kunderfranco@humanitasresearch.it
Organization name Humanitas Research Hospital
Department Research
Lab Bioinformatic Unit
Street address Via Rita Levi Montalcini 4
City Pieve Emanuele
State/province Milano
ZIP/Postal code 20090
Country Italy
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (7)
GSM3488812 Sham_1_week
GSM3488813 Sham_4_weeks_repA
GSM3488814 Sham_4_weeks_repB
Relations
BioProject PRJNA506965
SRA SRP170762

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122930_Dataset1-Average-clusters-conditions.xlsx 10.0 Mb (ftp)(http) XLSX
GSE122930_Dataset2-Markers-clusters-conditions.xlsx 4.8 Mb (ftp)(http) XLSX
GSE122930_Sham_1_week_barcodes.tsv.gz 10.5 Kb (ftp)(http) TSV
GSE122930_Sham_1_week_genes.tsv.gz 212.5 Kb (ftp)(http) TSV
GSE122930_Sham_1_week_matrix.mtx.gz 10.2 Mb (ftp)(http) MTX
GSE122930_Sham_4_weeks_repA+B_barcodes.tsv.gz 25.0 Kb (ftp)(http) TSV
GSE122930_Sham_4_weeks_repA+B_genes.tsv.gz 212.5 Kb (ftp)(http) TSV
GSE122930_Sham_4_weeks_repA+B_matrix.mtx.gz 19.0 Mb (ftp)(http) MTX
GSE122930_TAC_1_week_repA+B_barcodes.tsv.gz 19.8 Kb (ftp)(http) TSV
GSE122930_TAC_1_week_repA+B_genes.tsv.gz 212.5 Kb (ftp)(http) TSV
GSE122930_TAC_1_week_repA+B_matrix.mtx.gz 16.9 Mb (ftp)(http) MTX
GSE122930_TAC_4_weeks_repA+B_barcodes.tsv.gz 22.1 Kb (ftp)(http) TSV
GSE122930_TAC_4_weeks_repA+B_genes.tsv.gz 212.5 Kb (ftp)(http) TSV
GSE122930_TAC_4_weeks_repA+B_matrix.mtx.gz 21.0 Mb (ftp)(http) MTX
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Raw data are available in SRA
Processed data are available on Series record

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