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Series GSE122679 Query DataSets for GSE122679
Status Public on Nov 20, 2018
Title Receptor tyrosine kinase signaling promotes post-embryonic morphogenesis and survival of glia and neural progenitor cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Glioblastomas (GBMs), which are the most deadly malignant brain tumors of children and adults, infiltrate the brain and grow rapidly, and are resistant to current therapies. Glioblastomas (GBMs) frequently display mutations that activate receptor tyrosine kinases (RTKs), such as EGFR, which are thought to cooperate with additional factors to drive tumorigenesis. To identify and characterize genetic pathways that cooperate with RTKs to drive GBM progression, we performed RNA sequencing on several cell cultures created from surgical specimens from tumors with alterations in RTKs, and these cultures were grown as neurospheres in serum-free media used for normal neural stem/progenitor cell cultures. Under these conditions, GBM cells retain many defining features and mutations found in primary tumors. For comparison, we profiled commercially available human normal neural stem cells (HNPCs), which were grown under the same conditions. Data from these cell cultures was analyzed for transcripts differentially expressed between GBM cells and HNPCs, which showed that GBM cells that show RTK alterations strongly overexpressed the PDGFA and IGF2 secreted factors, which likely cooperate with EGFR to drive tumor progression.
 
Overall design Transcriptomic profiling of human glioblastoma neurospheres
 
Contributor(s) Read RD
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Submission date Nov 19, 2018
Last update date Mar 27, 2019
Contact name Renee Read
E-mail(s) renee.read@emory.edu
Phone 4047275985
Organization name Emory University School of Medicine
Department Pharmacology
Street address 1510 Clifton Rd
City Atlanta
State/province Georgia
ZIP/Postal code 30322
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (10)
GSM3478003 HNPC A
GSM3478004 HNPC B
GSM3478005 56 A
Relations
BioProject PRJNA506003
SRA SRP169574

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122679_cufflinks_HNPC_v_GBM_comparison.xlsx 8.2 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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